Cell Cycle Antibodies 1

NCAPH Rabbit Polyclonal Antibody (CAB4548)

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SKU:
CAB4548
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
Synonyms:
CAPH, BRRN1, CAP-H, MCPH23, NCAPH
Research Use:
Cell Cycle
Frequently bought together:

Description

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NCAPH Antibody (CAB4548)

The NCAPH Antibody (CAB4548) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it suitable for use in Western blot applications. By specifically binding to the Ncaph protein, this antibody enables precise detection and analysis in various cell types, making it ideal for studies in genetics, cell biology, and cancer research.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:NCAPH Antibody
SKU:CAB4548
Size:20μL, 100μL
Reactivity:Human
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MGPP GPAL PATM NNSS SETR GHPH SASS PSER VFPM PLPR KAPL NIPG TPVL EDFP QNDD EKER LQRR RSRV FDLQ FSTD SPRL LASP SSRS IDIS ATIP KFTN TQIT EHYS TCIK LSTE NKIT TKNA FGLH LIDF MSEI LKQK DTEP TNFK VAAG TLDA STKI YAVR VDAV HADV YRVL GGLG KDAP SLEE VEGH VADG SATE MGTT KKAV KPKK KHLH RTIE QNIN NLNV SEAD RKCE IDPM FQKT AA
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:5000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CAPH, BRRN1, CAP-H, MCPH23, NCAPH
Positive Sample:A-549, HepG2
Cellular Localization:Chromosome, Cytoplasm, Nucleus.
Calculated MW:83kDa
Observed MW:85kDa

This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. Alternatively spliced transcript variants encoding different proteins have been described.

Purification MethodAffinity purification
Gene ID23397
RRIDAB_2765747
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using NCAPH Rabbit pAb (CAB4548) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
Product ImageImmunofluorescence analysis of U2OS cells using NCAPH Rabbit pAb (CAB4548) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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