KO Validated Antibodies 1

[KO Validated] NBS1/NBN Rabbit Polyclonal Antibody (CAB0783)

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SKU:
CAB0783
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
Reactivity:
Human, Mouse, Rat
Synonyms:
ATV, NBS, P95, NBS1, AT-V1, AT-V2, BN
Research Use:
Cell Cycle
Frequently bought together:

Description

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[KO Validated] NBS1/NBN Antibody (CAB0783)

The [KO Validated] NBS1/NBN Antibody (CAB0783) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.The NBN protein, also known as nibrin, is part of the MRN complex involved in DNA double-strand break repair. Mutations in the NBN gene have been associated with a predisposition to certain types of cancer, making it a promising target for cancer research.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] NBS1/NBN Antibody
SKU:CAB0783
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:NNLF TDTD LKSI VKNS ASKS HAAE KLRS NKKR EMDD VAIE DEVL EQLF KDTK PELE IDVK VQKQ EEDV NVRK RPRM DIET NDTF SDEA VPES SKIS QENE IGKK RELK EDSL WSAK EISN NDKL QDDS EMLP KKLL LTEF RSLV IKNS TSRN PSGI NDDY GQLK NFKK FKKV TYPG AGKL PHII GGSD LIAH HARK NTEL EEWL RQEM EVQN QHAK EESL ADDL FRYN PYLK RRR
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ATV, NBS, P95, NBS1, AT-V1, AT-V2, BN
Positive Sample:293T, Mouse testis, Rat testis
Cellular Localization:Chromosome, Nucleus, Pml Body, Telomere.
Calculated MW:85kDa
Observed MW:95kDa/70kDa

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.

Purification MethodAffinity purification
Gene ID4683
RRIDAB_2757395
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and NBS1/NBS1/NBN knockout (KO) 293T cells, using [KO Validated] NBS1/NBN Rabbit pAb (CAB0783) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human lung cancer using [KO Validated] NBS1/NBS1/NBN Rabbit pAb (CAB0783) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
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