KO Validated Antibodies 2

Anti-MYL6B Antibody (CAB18102)[KO Validated]

(No reviews yet) Write a Review
SKU:
CAB18102
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-MYL6B Antibody [KO Validated]
Antibody SKU:CAB18102
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-208 of human MYL6B (NP_002466.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:500
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-208 of human MYL6B (NP_002466.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPPK KDVP VKKP AGPS ISKP AAKP AAAG APPA KTKA EPAV PQAP QKTQ EPPV DLSK VVIE FNKD QLEE FKEA FELF DRVG DGKI LYSQ CGDV MRAL GQNP TNAE VLKV LGNP KSDE LKSR RVDF ETFL PMLQ AVAK NRGQ GTYE DYLE GFRV FDKE GNGK VMGA ELRH VLTT LGEK MTEE EVET VLAG HEDS NGCI NYEA FLKH ILSV
Gene ID:140465
Uniprot:P14649
Cellular Location:
Calculated MW:22kDa
Observed MW:25kDa
Anti-MYL6B Antibody [KO Validated] (CAB18102)Western blot analysis of extracts from normal (control) and MYL6B knockout (KO) 293T cells, using MYL6B antibody (CAB18102) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Synonyms:MYL6B, MLC1SA
Background:Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in both slow-twitch skeletal muscle and in nonmuscle tissue. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:MYL6B: Regulatory light chain of myosin. Does not bind calcium.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Contractile

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cytosol; muscle myosin complex; myosin complex; unconventional myosin complex

Molecular Function:motor activity; protein binding; structural constituent of muscle

Biological Process: muscle contraction; muscle filament sliding; skeletal muscle development

NCBI Summary:Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in both slow-twitch skeletal muscle and in nonmuscle tissue. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
UniProt Code:P14649
NCBI GenInfo Identifier:127153
NCBI Gene ID:140465
NCBI Accession:P14649.1
UniProt Related Accession:P14649
Molecular Weight:22,764 Da
NCBI Full Name:Myosin light chain 6B
NCBI Synonym Full Names:myosin light chain 6B
NCBI Official Symbol:MYL6B  
NCBI Official Synonym Symbols:MLC1SA  
NCBI Protein Information:myosin light chain 6B
UniProt Protein Name:Myosin light chain 6B
UniProt Synonym Protein Names:Myosin light chain 1 slow-twitch muscle A isoform; MLC1sa; Smooth muscle and nonmuscle myosin light chain alkali 6B
Protein Family:Myosin
UniProt Gene Name:MYL6B  
UniProt Entry Name:MYL6B_HUMAN
View AllClose

0 Reviews

View AllClose

Related Products