Product Name:	Anti-Mouse IFNgamma [XMG1.2] In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0022
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	XMG1.2
Protein:	IFN-γ
Product Type:	Monoclonal Antibody
Synonyms:	Immune Interferon, Type II Interferon, T Cell Interferon, MAF, IFNG, IFG, IFI
Isotype:	Rat IgG1 κ
Reactivity:	Mouse
Immunogen:	Purified Recombinant Mouse IFN-γ (>98%)
Applications:	CyTOF®, ELISA Cap, ELISPOT, ICFC, IHC FF, N, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Applications:	CyTOF®, ELISA Cap, ELISPOT, ICFC, IHC FF, N, WB
Recommended Usage:	ELISA Cap XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA with a concentration range of 0.5-2.0 µg/ml as recommended. WB The suggested concentration for this XMG1.2 antibody for use in western blotting is 1-10 µg/ml.
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Rat Anti-Mouse Interferon Gamma (IFN-γ) (Clone XMG1.2) recognizes an epitope on Mouse IFN-γ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Immunogen:	Purified Recombinant Mouse IFN-γ (>98%)
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.3