Signal Transduction Antibodies 3

MFI2 Rabbit Polyclonal Antibody (CAB6653)

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SKU:
CAB6653
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction
Reactivity:
Human, Mouse, Rat
Synonyms:
MTf, MFI2, MTF1, CD228, MAP97
Research Use:
Signal Transduction
Frequently bought together:

Description

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MFI2 Antibody (CAB6653)

The MFI2 Antibody (CAB6653) is a high-quality antibody developed for reliable detection and analysis of target proteins. This high-quality antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications.Melanotransferrin, also known as MTF or CD228, is a glycoprotein that plays a role in iron metabolism and cellular growth regulation. It has been implicated in diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases, making it a target of interest in research aimed at understanding the mechanisms underlying these conditions.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:MFI2 Antibody
SKU:CAB6653
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:GMEV RWCA TSDP EQHK CGNM SEAF REAG IQPS LLCV RGTS ADHC VQLI AAQE ADAI TLDG GAIY EAGK EHGL KPVV GEVY DQEV GTSY YAVA VVRR SSHV TIDT LKGV KSCH TGIN RTVG WNVP VGYL VESG RLSV MGCD VLKA VSDY FGGS CVPG AGET SYSE SLCR LCRG DSSG EGVC D
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:MTf, MFI2, MTF1, CD228, MAP97
Positive Sample:293T, SW480, A375, Mouse liver, Mouse brain, Mouse thymus
Cellular Localization:Cell Membrane, Gpi-Anchor, Lipid-Anchor.
Calculated MW:80kDa
Observed MW:110kDa

The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants.

Purification MethodAffinity purification
Gene ID4241
RRIDAB_2767240
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using MFI2 Rabbit pAb (CAB6653) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunofluorescence analysis of L929 cells using MFI2 Rabbit pAb (CAB6653) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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