Name: Anti-METTL3 Antibody [KO Validated] (CAB19079)
Brand: KO Validated Antibodies 2
SKU: CAB19079
Availability: OutOfStock

Antibody Name:	Anti-METTL3 Antibody [KO Validated]
Antibody SKU:	CAB19079
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	A synthesized peptide derived from human METTL3

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa, Mouse brain, Mouse testis, 293T

Immunogen:	A synthesized peptide derived from human METTL3
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	56339
Uniprot:	Q86U44
Cellular Location:	Nucleus speckle
Calculated MW:	70kDa
Observed MW:	80kDa

	Western blot analysis of extracts from normal (control) and METTL3 knockout (KO) 293T cells, using METTL3 antibody (CAB19079) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
	Western blot analysis of extracts of various cell lines, using METTL3 antibody (CAB19079) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
	Immunofluorescence analysis of HeLa cells using METTL3 Rabbit mAb (CAB19079) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Synonyms:	IME4, M6A, MT-A70, Spo8, hMETTL3, METTL3
Background:	This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008]

UniProt Protein Function:	METTL3: N6-methyltransferase that methylates adenosine residues of some mRNAs. N6-methyladenosine (m6A), which is present at internal sites of some mRNAs, may play a role in the efficiency of mRNA splicing, transport or translation. Belongs to the MT-A70-like family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Methyltransferase; EC 2.1.1.62; RNA processing Chromosomal Location of Human Ortholog: 14q11.1 Cellular Component: nucleoplasm; nuclear speck; nucleus Molecular Function:mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity; RNA binding Biological Process: circadian rhythm; stem cell maintenance; RNA methylation; gene expression; mRNA processing
NCBI Summary:	This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008]
UniProt Code:	Q86U44
NCBI GenInfo Identifier:	21361827
NCBI Gene ID:	56339
NCBI Accession:	NP_062826.2
UniProt Secondary Accession:	Q86U44,O14736, Q86V05, Q9HB32,
UniProt Related Accession:	Q86U44
Molecular Weight:	25,479 Da
NCBI Full Name:	N6-adenosine-methyltransferase 70 kDa subunit
NCBI Synonym Full Names:	methyltransferase like 3
NCBI Official Symbol:	METTL3
NCBI Official Synonym Symbols:	M6A; IME4; Spo8; MT-A70
NCBI Protein Information:	N6-adenosine-methyltransferase 70 kDa subunit; mRNA m(6)A methyltransferase; methyltransferase-like protein 3; adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase
UniProt Protein Name:	N6-adenosine-methyltransferase 70 kDa subunit
UniProt Synonym Protein Names:	Methyltransferase-like protein 3
Protein Family:	N6-adenosine-methyltransferase
UniProt Gene Name:	METTL3
UniProt Entry Name:	MTA70_HUMAN