Antibody Name:	Anti-MERTK Antibody
Antibody SKU:	CAB5443
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 840-999 of human MERTK (NP_006334.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	22Rv1, Mouse liver, Mouse kidney, Mouse lung, Mouse spleen, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 840-999 of human MERTK (NP_006334.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DPLD RPTF SVLR LQLE KLLE SLPD VRNQ ADVI YVNT QLLE SSEG LAQG STLA PLDL NIDP DSII ASCT PRAA ISVV TAEV HDSK PHEG RYIL NGGS EEWE DLTS APSA AVTA EKNS VLPG ERLV RNGV SWSH SSML PLGS SLPD ELLF ADDS SEGS EVLM
Gene ID:	10461
Uniprot:	Q12866
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	110kDa
Observed MW:	160kDa

Synonyms:

MERTK, MER, RP38, Tyro12, c-Eyk, c-mer

Background:

This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP).

UniProt Protein Function:	Mer: a proto-oncogene receptor tyrosine-protein kinase of the Axl family. Binds several ligands including LGALS3, Tubby, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3
UniProt Protein Details:	Protein type:Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Oncoprotein; Kinase, protein; TK group; Axl family Chromosomal Location of Human Ortholog: 2q14.1 Cellular Component: extracellular space; photoreceptor outer segment; integral to plasma membrane; cytoplasm; plasma membrane Molecular Function:protein binding; transmembrane receptor protein tyrosine kinase activity; ATP binding Biological Process: platelet activation; negative regulation of lymphocyte activation; peptidyl-tyrosine phosphorylation; vagina development; protein amino acid phosphorylation; phagocytosis; cell surface receptor linked signal transduction; cell-cell signaling; retina development in camera-type eye; natural killer cell differentiation; positive regulation of phagocytosis; protein kinase B signaling cascade; spermatogenesis; blood coagulation; leukocyte migration; apoptotic cell clearance; secretion by cell Disease: Retinitis Pigmentosa 38
NCBI Summary:	This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP). [provided by RefSeq, Jul 2008]
UniProt Code:	Q12866
NCBI GenInfo Identifier:	160332297
NCBI Gene ID:	10461
NCBI Accession:	Q12866.2
UniProt Secondary Accession:	Q12866,Q9HBB4,
UniProt Related Accession:	Q12866
Molecular Weight:	999
NCBI Full Name:	Tyrosine-protein kinase Mer
NCBI Synonym Full Names:	MER proto-oncogene, tyrosine kinase
NCBI Official Symbol:	MERTK
NCBI Official Synonym Symbols:	MER; RP38; c-Eyk; c-mer; Tyro12
NCBI Protein Information:	tyrosine-protein kinase Mer; STK kinase; proto-oncogene c-Mer; MER receptor tyrosine kinase; receptor tyrosine kinase MerTK; c-mer proto-oncogene tyrosine kinase
UniProt Protein Name:	Tyrosine-protein kinase Mer
UniProt Synonym Protein Names:	Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK
Protein Family:	Tyrosine-protein kinase
UniProt Gene Name:	MERTK
UniProt Entry Name:	MERTK_HUMAN

	Western blot analysis of extracts of various cell lines, using MERTK antibody (CAB5443) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
	Immunofluorescence analysis of U2OS cells using MERTK antibody (CAB5443) at dilution of 1:100. Blue: DAPI for nuclear staining.