The Myoglobin Antibody (CAB5471) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human myoglobin and is optimized for use in Western blot applications. By binding to the myoglobin protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an ideal choice for studies in muscle physiology, cardiovascular health, and exercise science.Myoglobin is essential for muscle function, as it helps facilitate the delivery of oxygen to muscle cells during periods of increased activity.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Myoglobin Antibody
SKU:
CAB5471
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MYOSB, PVALB, Myoglobin
Positive Sample:
Mouse skeletal muscle, Rat skeletal muscle
Cellular Localization:
Cytosol, Extracellular Exosome.
Calculated MW:
17kDa
Observed MW:
17kDa
This gene encodes a member of the globin superfamily and is predominantly expressed in skeletal and cardiac muscles. The encoded protein forms a monomeric globular haemoprotein that is primarily responsible for the storage and facilitated transfer of oxygen from the cell membrane to the mitochondria. This protein also plays a role in regulating physiological levels of nitric oxide. Multiple transcript variants encoding distinct isoforms exist for this gene.
Purification Method
Affinity purification
Gene ID
4151
RRID
AB_2766272
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Myoglobin Rabbit pAb (CAB5471) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): Rat liver. Exposure time: 1s.
Immunofluorescence analysis of paraffin-embedded rat skeletal muscle using Myoglobin Rabbit pAb (CAB5471) at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
