Cell Biology Antibodies 13

Anti-MAP4K2 Antibody (CAB17524)

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SKU:
CAB17524
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-MAP4K2 Antibody
Antibody SKU:CAB17524
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 551-820 of human MAP4K2 (NP_004570.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, A-549, C6, Raji, HeLa, Mouse lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 551-820 of human MAP4K2 (NP_004570.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:WAHD LPGL FEQR RLQQ QVPL SIPT NRLT QRII PRRF ALST KIPD TKGC LQCR VVRN PYTG ATFL LAAL PTSL LLLQ WYEP LQKF LLLK NFSS PLPS PAGM LEPL VLDG KELP QVCV GAEG PEGP GCRV LFHV LPLE AGLT PDIL IPPE GIPG SAQQ VIQV DRDT ILVS FERC VRIV NMQG EPTA TLAP ELTF DFPI ETVV CLQD SVLA FWSH GMQG RSLD TNEV TQEI TDET RIFR VLGA HRDI ILES IPTD NPEA HSNL YILT GHQS TY
Gene ID:5871
Uniprot:Q12851
Cellular Location:
Calculated MW:
Observed MW:110kDa
Anti-MAP4K2 Antibody (CAB17524)Western blot - MAP4K2 Polyclonal Antibody (CAB17524)
Synonyms:BL44, GCK, RAB8IP, MAP4K2
Background:The protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
UniProt Protein Function:GCK: Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extend of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation. The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3. May play a role in the regulation of vesicle targeting or fusion. regulation of vesicle targeting or fusion. Interacts with TRAF2, TRAF6, MAP3K1/MEKK1 and MAP3K11/MLK3. Interacts with RAB8A. Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined. The tumor necrosis factor (TNF), as well as endotoxins and proinflammatory stimuli such as polyinosine- polycytidine (poly(IC)), lipopolysaccharides (LPS), peptidoglycan (PGN), flagellin, or lipid A activate MAP4K2 by promoting its autophosphorylation. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Protein kinase, STE; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; STE group; STE20 family; KHS subfamily

Chromosomal Location of Human Ortholog: 11q13

Molecular Function:ATP binding; mitogen-activated protein kinase kinase kinase binding; protein binding; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: activation of JNK activity; immune response; JNK cascade; positive regulation of JNK cascade; protein amino acid phosphorylation

NCBI Summary:The protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
UniProt Code:Q12851
NCBI GenInfo Identifier:215274019
NCBI Gene ID:5871
NCBI Accession:Q12851.2
UniProt Secondary Accession:Q12851,Q86VU3,
UniProt Related Accession:Q12851
Molecular Weight:90,809 Da
NCBI Full Name:Mitogen-activated protein kinase kinase kinase kinase 2
NCBI Synonym Full Names:mitogen-activated protein kinase kinase kinase kinase 2
NCBI Official Symbol:MAP4K2  
NCBI Official Synonym Symbols:GCK; BL44; RAB8IP  
NCBI Protein Information:mitogen-activated protein kinase kinase kinase kinase 2
UniProt Protein Name:Mitogen-activated protein kinase kinase kinase kinase 2
UniProt Synonym Protein Names:B lymphocyte serine/threonine-protein kinase; Germinal center kinase; GC kinase; MAPK/ERK kinase kinase kinase 2; MEK kinase kinase 2; MEKKK 2; Rab8-interacting protein
Protein Family:Mitogen-activated protein kinase kinase kinase kinase
UniProt Gene Name:MAP4K2  
UniProt Entry Name:M4K2_HUMAN
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