Epigenetics & Nuclear Signaling Antibodies 3

LRPPRC Rabbit Polyclonal Antibody (CAB3365)

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SKU:
CAB3365
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
LSFC, GP130, LRP130, MC4DN5, CLONE-23970, LRPPRC
Research Use:
Epigenetics and Nuclear Signaling
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Description

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LRPPRC Antibody (CAB3365)

The LRPPRC Antibody (CAB3365) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human LRPPRC samples and has been extensively validated for Western blot applications.LRPPRC, also known as leucine rich PPR motif-containing protein, is essential for maintaining mitochondrial function and energy production in cells. Dysregulation of LRPPRC has been implicated in various diseases including metabolic disorders and cancer.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:LRPPRC Antibody
SKU:CAB3365
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:IACR LNQK KGAY DIFL NAKE QNIV FNAE TYSN LIKL LMSE DYFT QAME VKAF AETH IKGF TLND AANS RLII TQVR RDYL KEAV TTLK TVLD QQQT PSRL AVTR VIQA LAMK GDVE NIEV VQKM LNGL EDSI GLSK MVFI NNIA LAQI KNNN IDAA IENI ENML TSEN KVIE PQYF GLAY LFRK VIEE QLEP AVEK ISIM AERL ANQF AIYK PVTD FFLQ LVDA GKVD DARA LLQR CGAI AEQT PILL LFLL RNSR KQGK ASTV KSVL ELIP ELNE KEEA YNSL MKSY VSEK DVTS AKAL YEHL TAKN TKLD DLFL KRYA SLLK YAGE PVPF IEPP ESFE FYAQ QLRK LREN SS
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:1000 - 1:5000
IHC-P1:50 - 1:200
IF/ICC1:100 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:LSFC, GP130, LRP130, MC4DN5, CLONE-23970, LRPPRC
Positive Sample:HepG2, HeLa, Mouse heart, Rat heart, Rat brain
Cellular Localization:Mitochondrion, Nucleus, Nucleus Inner Membrane, Nucleus Outer Membrane, Nucleoplasm.
Calculated MW:158kDa
Observed MW:158kDa

This gene encodes a leucine-rich protein that has multiple pentatricopeptide repeats (PPR). The precise role of this protein is unknown but studies suggest it may play a role in cytoskeletal organization, vesicular transport, or in transcriptional regulation of both nuclear and mitochondrial genes. The protein localizes primarily to mitochondria and is predicted to have an N-terminal mitochondrial targeting sequence. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome.

Purification MethodAffinity purification
Gene ID10128
RRIDAB_2765075
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using LRPPRC Rabbit pAb (CAB3365) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 0.5s.
Product ImageWestern blot analysis of various lysates, using LRPPRC Rabbit pAb (CAB3365) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon carcinoma using LRPPRC Rabbit pAb (CAB3365) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageConfocal immunofluorescence analysis of Hela cells using LRPPRC Rabbit pAb (CAB3365) at dilution of 1:400. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of HepG2 cells using LRPPRC Rabbit pAb (CAB3365) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using LRPPRC Rabbit pAb (CAB3365) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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