Cell Biology Antibodies 1

LOXL1 Rabbit Polyclonal Antibody (CAB10191)

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SKU:
CAB10191
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse
Synonyms:
LOL, LOXL, LOXL1
Research Use:
Cell Biology
Frequently bought together:

Description

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LOXL1 Antibody (CAB10191)

The LOXL1 Antibody (CAB10191) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and demonstrates high specificity towards human samples, making it an excellent tool for use in Western blot applications. By binding to the LOXL1 protein, researchers can accurately detect and study its expression in various cell types, making it an invaluable asset for investigations in the fields of molecular biology, tissue engineering, and fibrosis research.LOXL1, also known as lysyl oxidase-like 1, plays a crucial role in maintaining tissue integrity and regulating collagen cross-linking, essential processes for overall tissue health and function.

This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:LOXL1 Antibody
SKU:CAB10191
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:RQAP SLPL PGRV GSDT VRGQ ARHP FGFG QVPD NWRE VAVG DSTG MARA RTSV SQQR HGGS ASSV SASA FAST YRQQ PSYP QQFP YPQA PFVS QYEN YDPA SRTY DQGF VYYR PAGG GVGA GAAA VASA GVIY PYQP RARY EEYG GGEE LPEY PPQG FYPA PERP YVPP PPPP PDGL DRRY SHSL YSEG TPGF EQAY PDPG PEAA QAHG GDPR LGWY PPYA NPPP EAYG PPRA LEPP YLPV RSSD TPPP GGER NGAQ QGRL SVGS VYRP NQNG RGLP
Tested Applications:WB ELISA IF-P
Recommended Dilution:
WB1:500 - 1:1000
IF-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:LOL, LOXL, LOXL1
Positive Sample:HeLa, A375, A-549, BT-474, HT-1080, Mouse lung, Mouse heart, Rat heart, U-87 MG
Cellular Localization:Secreted, Extracellular Space.
Calculated MW:63kDa
Observed MW:80kDa/63kDa

This gene encodes a member of the lysyl oxidase family of proteins. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyzes the first step in the formation of crosslinks in collagen and elastin. The encoded preproprotein is proteolytically processed to generate the mature enzyme. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. Mutations in this gene are associated with exfoliation syndrome.

Purification MethodAffinity purification
Gene ID4016
RRIDAB_2757713
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using LOXL1 Rabbit pAb (CAB10191)at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): U-937 Exposure time: 30s.
Product ImageImmunofluorescence analysis of paraffin-embedded mouse eye using LOXL1 Rabbit pAb (CAB10191) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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