KO Validated Antibodies 1

[KO Validated] LITAF Rabbit Polyclonal Antibody (CAB5469)

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SKU:
CAB5469
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse
Synonyms:
PIG7, SIMPLE, TP53I7, AF
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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[KO Validated] LITAF Antibody (CAB5469)

The [KO Validated] LITAF Antibody (CAB5469) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reliable for use in various research applications, particularly in immunology and cancer studies. It is validated for Western blotting, enabling the detection and analysis of LITAF protein in human samples and various cell types.LITAF, also known as lipopolysaccharide-induced TNF-alpha factor, is a key player in the regulation of inflammatory responses and immune activation.

This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:[KO Validated] LITAF Antibody
SKU:CAB5469
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MSVP GPYQ AATG PSSA PSAP PSYE ETVA VNSY YPTP PAPM PGPT TGLV TGPD GKGM NPPS YYTQ PAPI PNNN PITV QTVY VQHP ITFL DRPI QMCC PSCN KMIV SQLS YNAG ALTW LSCG SLCL LGCI AGCC FIPF CVDA LQDV DHYC PNCR ALLG TYKR L
Tested Applications:WB IHC-P IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PIG7, SIMPLE, TP53I7, AF
Positive Sample:HeLa
Cellular Localization:Cytoplasmic Side, Lysosome Membrane, Peripheral Membrane Protein.
Calculated MW:17kDa
Observed MW:23kDa

Lipopolysaccharide is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor-alpha (TNF-alpha) and other inflammatory mediators. This gene encodes lipopolysaccharide-induced TNF-alpha factor, which is a DNA-binding protein and can mediate the TNF-alpha expression by direct binding to the promoter region of the TNF-alpha gene. The transcription of this gene is induced by tumor suppressor p53 and has been implicated in the p53-induced apoptotic pathway. Mutations in this gene cause Charcot-Marie-Tooth disease type 1C (CMT1C) and may be involved in the carcinogenesis of extramammary Paget's disease (EMPD). Multiple alternatively spliced transcript variants have been found for this gene.

Purification MethodAffinity purification
Gene ID9516
RRIDAB_2766270
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and LITAF knockout (KO) HeLa cells, using [KO Validated] LITAF Rabbit pAb (CAB5469) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageWestern blot analysis of lysates from HeLa cells, using LITAF Rabbit pAb (CAB5469) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
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