Epigenetics & Nuclear Signaling Antibodies 4

Ku70 Rabbit Polyclonal Antibody (CAB7330)

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SKU:
CAB7330
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
ML8, KU70, TLAA, CTC75, CTCBF, G22P1, Ku70
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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Ku70 Antibody (CAB7330)

The Ku70 Antibody (CAB7330) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By binding to the XRCC6 protein, this antibody allows for accurate detection and analysis in various cell types, making it an ideal choice for studies in genetics, cancer research, and radiation biology.XRCC6, a subunit of the Ku protein complex, plays a crucial role in non-homologous end joining, a DNA repair pathway essential for the repair of double-strand breaks.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Ku70 Antibody
SKU:CAB7330
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:EQAV DLTL PKVE AMNK RLGS LVDE FKEL VYPP DYNP EGKV TKRK HDNE GSGS KRPK VEYS EEEL KTHI SKGT LGKF TVPM LKEA CRAY GLKS GLKK QELL
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:100
IF/ICC1:50 - 1:100
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ML8, KU70, TLAA, CTC75, CTCBF, G22P1, Ku70
Positive Sample:COS-7, SW480, Mouse brain
Cellular Localization:Chromosome, Nucleus.
Calculated MW:70kDa
Observed MW:70kDa

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.

Purification MethodAffinity purification
Gene ID2547
RRIDAB_2767867
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Ku70 Rabbit pAb (CAB7330) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat brain using Ku70 Rabbit pAb (CAB7330) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human uterus using Ku70 Rabbit pAb (CAB7330) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of A549 cells using Ku70 Rabbit pAb (CAB7330). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunoprecipitation analysis of 100 μg extracts of SW480 cells using 3 μg Ku70 antibody (CAB7330). Western blot was performed from the immunoprecipitate using Ku70 antibody (CAB7330) at a dilution of 1:1000.
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