KO Validated Antibodies 1

Anti-KDM5B Antibody (CAB7772)[KO Validated]

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SKU:
CAB7772
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-KDM5B Antibody [KO Validated]
Antibody SKU:CAB7772
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 784-883 of human KDM5B (NP_006609.3).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 784-883 of human KDM5B (NP_006609.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EESE MKKF PDND LLRH LRLV TQDA EKCA SVAQ QLLN GKRQ TRYR SGGG KSQN QLTV NELR QFVT QLYA LPCV LSQT PLLK DLLN RVED FQQH SQKL LSEE
Gene ID:10765
Uniprot:Q9UGL1
Cellular Location:Nucleus
Calculated MW:175kDa/179kDa
Observed MW:176KDa
Synonyms:KDM5B, CT31, JARID1B, PLU-1, PLU1, PPP1R98, PUT1, RBBP2H1A, RBP2-H1
Background:This gene encodes a lysine-specific histone demethylase that belongs to the jumonji/ARID domain-containing family of histone demethylases. The encoded protein is capable of demethylating tri-, di- and monomethylated lysine 4 of histone H3. This protein plays a role in the transcriptional repression or certain tumor suppressor genes and is upregulated in certain cancer cells. This protein may also play a role in genome stability and DNA repair. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:JARID1B: Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma. Interacts with FOXG1B, PAX9, MYC, MYCN and RB1. Interacts with HDAC1, HDAC4, HDAC5 and HDAC7. Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer. Belongs to the JARID1 histone demethylase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Demethylase; Cancer Testis Antigen (CTA); EC 1.14.11.-; Oxidoreductase; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function:histone demethylase activity; histone demethylase activity (H3-K4 specific); protein binding; transcription corepressor activity; transcription factor activity

NCBI Summary:This gene encodes a lysine-specific histone demethylase that belongs to the jumonji/ARID domain-containing family of histone demethylases. The encoded protein is capable of demethylating tri-, di- and monomethylated lysine 4 of histone H3. This protein plays a role in the transcriptional repression or certain tumor suppressor genes and is upregulated in certain cancer cells. This protein may also play a role in genome stability and DNA repair. Alternate splicing resultsi n multiple transcript variants. [provided by RefSeq, Sep 2015]
UniProt Code:Q9UGL1
NCBI GenInfo Identifier:296439317
NCBI Gene ID:10765
NCBI Accession:Q9UGL1.3
UniProt Secondary Accession:Q9UGL1,O95811, Q15752, Q9Y3Q5,
UniProt Related Accession:Q9UGL1
Molecular Weight:179,409 Da
NCBI Full Name:Lysine-specific demethylase 5B
NCBI Synonym Full Names:lysine demethylase 5B
NCBI Official Symbol:KDM5B  
NCBI Official Synonym Symbols:CT31; PLU1; PUT1; PLU-1; JARID1B; PPP1R98; RBP2-H1; RBBP2H1A  
NCBI Protein Information:lysine-specific demethylase 5B
UniProt Protein Name:Lysine-specific demethylase 5B
UniProt Synonym Protein Names:Cancer/testis antigen 31; CT31; Histone demethylase JARID1B; Jumonji/ARID domain-containing protein 1B; PLU-1; Retinoblastoma-binding protein 2 homolog 1; RBP2-H1
Protein Family:Lysine-specific demethylase
UniProt Gene Name:KDM5B  
UniProt Entry Name:KDM5B_HUMAN
Anti-KDM5B Antibody [KO Validated] (CAB7772)Western blot analysis of extracts of 293T cells, using KDM5B antibody (CAB7772) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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