Antibody Name:	Anti-KCND3 Antibody
Antibody SKU:	CAB6927
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 502-636 of human KCND3 (NP_751948.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Mouse brain, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 502-636 of human KCND3 (NP_751948.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MESS MQNY PSTR SPSL SSHP GLTT TCCS RRSK KTTH LPNS NLPA TRLR SMQE LSTI HIQG SEQP SLTT SRSS LNLK ADDG LRPN CKTS QITT AIIS IPTP PALT PEGE SRPP PASP GPNT NIPS IASN VVKV SAL
Gene ID:	3752
Uniprot:	Q9UK17
Cellular Location:	Cell membrane, Cell projection, Membrane, Multi-pass membrane protein, dendrite, sarcolemma
Calculated MW:	71kDa/73kDa
Observed MW:	73kDa

Synonyms:

KCND3, BRGDA9, KCND3L, KCND3S, KSHIVB, KV4.3, SCA19, SCA22

Background:

Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shal-related subfamily, members of which form voltage-activated A-type potassium ion channels and are prominent in the repolarization phase of the action potential. This member includes two isoforms with different sizes, which are encoded by alternatively spliced transcript variants of this gene.

UniProt Protein Function:	Kv4.3: Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits. KCND3 rare variants may confer risk for lethal ventricular arrhytmias and be associated with autopsy-negative sudden unexplained death syndrome (SUDS). Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.3/KCND3 sub-subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Membrane protein, multi-pass; Channel, potassium Chromosomal Location of Human Ortholog: 1p13.3 Cellular Component: voltage-gated potassium channel complex; cell soma; dendrite; plasma membrane; sarcolemma Molecular Function:protein binding; metal ion binding; delayed rectifier potassium channel activity; A-type (transient outward) potassium channel activity Biological Process: synaptic transmission; potassium ion transport; protein homooligomerization Disease: Brugada Syndrome 9; Spinocerebellar Ataxia 19
NCBI Summary:	Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shal-related subfamily, members of which form voltage-activated A-type potassium ion channels and are prominent in the repolarization phase of the action potential. This member includes two isoforms with different sizes, which are encoded by alternatively spliced transcript variants of this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UK17
NCBI GenInfo Identifier:	92090984
NCBI Gene ID:	3752
NCBI Accession:	Q9UK17.3
UniProt Secondary Accession:	Q9UK17,O60576, O60577, Q14D71, Q5T0M0, Q9UH85, Q9UH86 Q9UK16,
UniProt Related Accession:	Q9UK17
Molecular Weight:	655
NCBI Full Name:	Potassium voltage-gated channel subfamily D member 3
NCBI Synonym Full Names:	potassium voltage-gated channel, Shal-related subfamily, member 3
NCBI Official Symbol:	KCND3
NCBI Official Synonym Symbols:	KV4.3; SCA19; SCA22; KCND3L; KCND3S; KSHIVB
NCBI Protein Information:	potassium voltage-gated channel subfamily D member 3; voltage-gated K+ channel; potassium ionic channel Kv4.3; sha1-related potassium channel Kv4.3; voltage-gated potassium channel subunit Kv4.3
UniProt Protein Name:	Potassium voltage-gated channel subfamily D member 3
UniProt Synonym Protein Names:	Voltage-gated potassium channel subunit Kv4.3
Protein Family:	Potassium voltage-gated channel subfamily
UniProt Gene Name:	KCND3
UniProt Entry Name:	KCND3_HUMAN

	Western blot analysis of extracts of various cell lines, using KCND3 antibody (CAB6927) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse brain using KCND3 antibody (CAB6927) at dilution of 1:100 (40x lens).