Cell Cycle Antibodies 2

IST1 Rabbit Polyclonal Antibody (CAB9401)

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SKU:
CAB9401
Product Type:
Antibody
Applications:
WB
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
OLC1, CHMP8, IST1
Research Use:
Cell Cycle
Frequently bought together:

Description

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IST1 Antibody (CAB9401)

The IST1 Antibody (CAB9401) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit immunization, exhibits high specificity for IST1 in human samples and has been validated for use in Western blot applications. By binding to the IST1 protein, this antibody enables accurate detection and analysis of IST1 in various cell types, making it ideal for investigations in cell biology and molecular biology.IST1 is a key player in cell division and vesicle trafficking, making it a crucial target for research on cellular processes and organelle dynamics.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:IST1 Antibody
SKU:CAB9401
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MLGS GFKA ERLR VNLR LVIN RLKL LEKK KTEL AQKA RKEI ADYL AAGK DERA RIRV EHII REDY LVEA MEIL ELYC DLLL ARFG LIQS MKEL DSGL AESV STLI WAAP RLQS EVAE LKIV ADQL CAKY SKEY GKLC RTNQ IGTV NDRL MHKL SVEA PPKI LVER YLIE IAKN YNVP YEPD SVVM AEAP PGVE TDLI DVGF TDDV KKGG PGRG GSGG FTAP VGGP DGTV PMPM PMPM PMPS ANTP FSYP LPKG PVDD INAD KNIS SAQI VGPG PKPE ASAK LPSR PADN YDNF VLPE LPSV PDTL PTAS AGAS TSAS EDID FDDL SRRF EELK KKT
Tested Applications:WB ELISA
Recommended Dilution:
WB1:1000 - 1:5000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:OLC1, CHMP8, IST1
Positive Sample:A-549, HeLa, Mouse heart, Rat brain
Cellular Localization:Cytoplasm, Cytoplasmic Vesicle, Midbody, Centrosome, Cytoskeleton, Microtubule Organizing Center.
Calculated MW:40kDa
Observed MW:40kDa

This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Purification MethodAffinity purification
Gene ID9798
RRIDAB_2770001
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using IST1 Rabbit pAb (CAB9401) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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