The IRF2 Monoclonal Antibody (CAB4843) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in a variety of applications, including Western blotting.IRF2 plays a crucial role in the modulation of immune responses and has been implicated in various diseases, including cancer and autoimmune disorders.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
IRF2 Monoclonal Antibody
SKU:
CAB4843
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC0265
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IRF-2, IRF2
Positive Sample:
HeLa, Ramos, A20
Cellular Localization:
Nucleus.
Calculated MW:
39kDa
Observed MW:
50kDa
IRF2 encodes interferon regulatory factor 2, a member of the interferon regulatory transcription factor (IRF) family. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4.
Purification Method
Affinity purification
Gene ID
3660
RRID
AB_2863358
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using IRF2 Rabbit mAb (CAB4843) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg IRF2 antibody (CAB4843). Western blot was performed from the immunoprecipitate using IRF2 (CAB4843) at a dilution of 1:500.
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