Metabolism Antibodies 2

Anti-IREB1 Antibody (CAB7867)

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SKU:
CAB7867
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-IREB1 Antibody
Antibody SKU:CAB7867
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human IREB1 (NP_002188.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human IREB1 (NP_002188.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSNP FAHL AEPL DPVQ PGKK FFNL NKLE DSRY GRLP FSIR VLLE AAIR NCDE FLVK KQDI ENIL HWNV TQHK NIEV PFKP ARVI LQDF TGVP AVVD FAAM RDAV KKLG GDPE KINP VCPA DLVI DHSI QVDF NRRA DSLQ KNQD LEFE RNRE RFEF LKWG SQAF HNMR II
Gene ID:48
Uniprot:P21399
Cellular Location:Cytoplasm
Calculated MW:98kDa
Observed MW:Refer to figures
Synonyms:ACO1, ACONS, HEL60, IREB1, IREBP, IREBP1, IRP1
Background:The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants
UniProt Protein Function:IREB1: Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding. Belongs to the aconitase/IPM isomerase family.
UniProt Protein Details:

Protein type:EC 4.2.1.3; Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - glyoxylate and dicarboxylate; RNA-binding; Lyase; Endoplasmic reticulum; Translation

Chromosomal Location of Human Ortholog: 9p21.1

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; Golgi apparatus; mitochondrion

Molecular Function:4 iron, 4 sulfur cluster binding; aconitate hydratase activity; iron-responsive element binding; protein binding; RNA binding

Biological Process: citrate metabolic process; response to iron(II) ion

NCBI Summary:The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]
UniProt Code:P21399
NCBI GenInfo Identifier:3123225
NCBI Gene ID:48
NCBI Accession:P21399.3
UniProt Secondary Accession:P21399,Q14652, Q5VZA7, D3DRK7,
UniProt Related Accession:P21399
Molecular Weight:98,399 Da
NCBI Full Name:Cytoplasmic aconitate hydratase
NCBI Synonym Full Names:aconitase 1
NCBI Official Symbol:ACO1  
NCBI Official Synonym Symbols:IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1  
NCBI Protein Information:cytoplasmic aconitate hydratase
UniProt Protein Name:Cytoplasmic aconitate hydratase
UniProt Synonym Protein Names:Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1
Protein Family:Aconitate hydratase
UniProt Gene Name:ACO1  
UniProt Entry Name:ACOC_HUMAN
Anti-IREB1 Antibody (CAB7867)Immunohistochemistry of paraffin-embedded human prostate using IREB1 antibody (CAB7867) at dilution of 1:100 (40x lens).
Anti-IREB1 Antibody (CAB7867)Immunohistochemistry of paraffin-embedded rat kidney using IREB1 antibody (CAB7867) at dilution of 1:100 (40x lens).
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