Cell Biology Antibodies 10

INPP5J Rabbit Polyclonal Antibody (CAB6631)

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SKU:
CAB6631
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
PIPP, INPP5, PIB5PA, INPP5J
Research Use:
Cell Biology
Frequently bought together:

Description

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INPP5J Antibody (CAB6631)

The INPP5J Antibody (CAB6631) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity for human samples and has been validated for use in Western blot techniques. By targeting the INPP5J protein, this antibody allows for the detection and analysis of INPP5J in various cell types, making it suitable for studies in cell biology and signal transduction research. INPP5J is a phosphoinositide 5-phosphatase that plays a crucial role in regulating intracellular signaling cascades and membrane trafficking processes.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:INPP5J Antibody
SKU:CAB6631
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:VTWN VGTA MPPD DVTS LLHL GGGD DSDG ADMI AIGL QEVN SMLN KRLK DALF TDQW SELF MDAL GPFN FVLV SSVR MQGV ILLL FAKY YHLP FLRD VQTD CTRT GLGG YWGN KGGV SVRL AAFG HMLC FLNC HLPA HMDK AEQR KDNF QTIL SLQQ FQGP GAQG ILDH DLVF WFGD LNFR IESY DLHF VKFA IDSD QLHQ LWEK DQLN MAKN TWPI LKGF QEGP LNFA PTFK FDVG TNKY DTSA KKRK PAWT DRIL WKVK APGG GPSP SGRK SHRL QVTQ HSYR SHME YTVS DHKP VAAQ F
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:10 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PIPP, INPP5, PIB5PA, INPP5J
Positive Sample:A-549, A375
Cellular Localization:Cytoplasm.
Calculated MW:107kDa
Observed MW:107kDa

Predicted to enable phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity and phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity. Predicted to be involved in inositol phosphate dephosphorylation; negative regulation of peptidyl-serine phosphorylation; and phosphatidylinositol dephosphorylation. Predicted to act upstream of or within negative regulation of neuron projection development. Located in cytoplasm and ruffle.

Purification MethodAffinity purification
Gene ID27124
RRIDAB_2767220
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using INPP5J Rabbit pAb (CAB6631) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunofluorescence analysis of U2OS cells using INPP5J Rabbit pAb (CAB6631). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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