Anti-HUWE1 Antibody (CAB20708)
- SKU:
- CAB20708
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-HUWE1 Antibody (CAB20708) |
| Antibody SKU: | CAB20708 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HUWE1 (NP_113584.3). |
| Application: | Western blotting |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Rat |
| Positive Samples: | SKOV3, Rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HUWE1 (NP_113584.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MKVD RTKL KKTP TEAP ADCR ALID KLKV CNDE QLLL ELQQ IKTW NIGK CELY HWVD LLDR FDGI LADA GQTV ENMS WMLV CDRP EREQ LKML LLAV LNFT |
| Cellular Location: | |
| Calculated MW: | 481KDa |
| Observed MW: | 480KDa |
| Synonyms: | MULE, Ib772, LASU1, MRXST, UREB1, HECTH9, URE-B1, ARF-BP1, HSPC272 |
| Background: | This gene encodes a protein containing a C-terminal HECT (E6AP type E3 ubiquitin protein ligase) domain that functions as an E3 ubiquitin ligase. The encoded protein is required for the ubiquitination and subsequent degradation of the anti-apoptotic protein Mcl1 (myeloid cell leukemia sequence 1 (BCL2-related)). This protein also ubiquitinates the p53 tumor suppressor, core histones, and DNA polymerase beta. Mutations in this gene are associated with Turner type X-linked syndromic cognitive disability. |
 | Western blot analysis of extracts of various cell lines, using HUWE1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s. |
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