Anti-HUWE1 Antibody (CAB20708)

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SKU:
CAB20708
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Description

Antibody Name:Anti-HUWE1 Antibody (CAB20708)
Antibody SKU:CAB20708
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HUWE1 (NP_113584.3).
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Rat
Positive Samples:SKOV3, Rat brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HUWE1 (NP_113584.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MKVD RTKL KKTP TEAP ADCR ALID KLKV CNDE QLLL ELQQ IKTW NIGK CELY HWVD LLDR FDGI LADA GQTV ENMS WMLV CDRP EREQ LKML LLAV LNFT
Cellular Location:
Calculated MW:481KDa
Observed MW:480KDa
Synonyms:MULE, Ib772, LASU1, MRXST, UREB1, HECTH9, URE-B1, ARF-BP1, HSPC272
Background:This gene encodes a protein containing a C-terminal HECT (E6AP type E3 ubiquitin protein ligase) domain that functions as an E3 ubiquitin ligase. The encoded protein is required for the ubiquitination and subsequent degradation of the anti-apoptotic protein Mcl1 (myeloid cell leukemia sequence 1 (BCL2-related)). This protein also ubiquitinates the p53 tumor suppressor, core histones, and DNA polymerase beta. Mutations in this gene are associated with Turner type X-linked syndromic cognitive disability.
Western blot analysis of extracts of various cell lines, using HUWE1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Western blot analysis of extracts of various cell lines, using HUWE1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
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Additional Information

Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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