KO Validated Antibodies 2

[KO Validated] HuR/ELAVL1 Rabbit Monoclonal Antibody (CAB19622)

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SKU:
CAB19622
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Monoclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
HUR, Hua, MelG, ELAV1, L1
Research Use:
Cell Biology
Frequently bought together:

Description

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[KO Validated] HuR/ELAVL1 Monoclonal Antibody (CAB19622)

The [KO Validated] HuR/ELAVL1 Monoclonal Antibody (CAB19622) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and has been rigorously validated for knockout (KO) studies, ensuring reliable results.HUR/ELAVL1, also known as HuR, is a RNA-binding protein involved in mRNA stabilization and translation. Its dysregulation has been linked to various diseases, including cancer and inflammatory disorders, making it a promising target for therapeutic interventions. This antibody enables precise detection and analysis of HUR/ELAVL1 in different cell types, paving the way for deeper insights into its function and potential therapeutic applications.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] HuR/ELAVL1 Monoclonal Antibody
SKU:CAB19622
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC0101
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MSNG YEDH MAED CRGD IGRT NLIV NYLP QNMT QDEL RSLF SSIG EVES AKLI RDKV AGHS LGYG FVNY VTAK DAER AINT LNGL RLQS KTIK VSYA RPSS
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:
WB1:1000 - 1:6000
IHC-P1:200 - 1:2000
IF/ICC1:100 - 1:1000
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HUR, Hua, MelG, ELAV1, L1
Positive Sample:293T, HeLa, Jurkat, Mouse brain, Mouse spleen, Mouse thymus, Rat brain
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:36kDa
Observed MW:30kDa

The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy.

Purification MethodAffinity purification
Gene ID1994
RRIDAB_2862702
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and HuR/ELAVL1 knockout (KO) 293T cells, using [KO Validated] HuR/ELAVL1 Rabbit mAb (CAB19622) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageWestern blot analysis of various lysates using [KO Validated] HuR/ELAVL1 Rabbit mAb (CAB19622) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human kidney using [KO Validated] HuR/ELAVL1 Rabbit mAb (CAB19622) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human tonsil using [KO Validated] HuR/ELAVL1 Rabbit mAb (CAB19622) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageConfocal imaging of NIH/3T3 cells using [KO Validated] HuR/ELAVL1 Rabbit mAb (CAB19622,dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Product ImageConfocal imaging of U-2 OS cells using [KO Validated] HuR/ELAVL1 Rabbit mAb (CAB19622,dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
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