KO Validated Antibodies 1

[KO Validated] HuR/ELAVL1 Rabbit Polyclonal Antibody (CAB6089)

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SKU:
CAB6089
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse
Synonyms:
HUR, Hua, MelG, ELAV1, L1
Research Use:
Cell Biology
Frequently bought together:

Description

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[KO Validated] HuR/ELAVL1 Antibody (CAB6089)

The [KO Validated] HuR/ELAVL1 Antibody (CAB6089) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to ELAVL1 and is validated for use in various applications, including Western blot and immunofluorescence.ELAVL1, also known as Hu antigen R (HuR), plays a crucial role in post-transcriptional regulation of gene expression by binding to target mRNAs and stabilizing them. Dysregulation of ELAVL1 has been linked to numerous diseases, including cancer, neurodegenerative disorders, and inflammatory conditions.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:[KO Validated] HuR/ELAVL1 Antibody
SKU:CAB6089
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MSNG YEDH MAED CRGD IGRT NLIV NYLP QNMT QDEL RSLF SSIG EVES AKLI RDKV AGHS LGYG FVNY VTAK DAER AINT LNGL RLQS KTIK VSYA RPSS
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HUR, Hua, MelG, ELAV1, L1
Positive Sample:HeLa, MCF-7, Jurkat, 293T, Mouse spleen, Mouse thymus
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:36kDa
Observed MW:36kDa

The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy.

Purification MethodAffinity purification
Gene ID1994
RRIDAB_2863522
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] HuR/ELAVL1 Rabbit pAb (CAB6089) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Product ImageWestern blot analysis of lysates from wild type (WT) and HuR/ELAVL1 knockout (KO) 293T cells, using [KO Validated] HuR/ELAVL1 Rabbit pAb (CAB6089) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human stomach using [KO Validated] HuR/ELAVL1 Rabbit pAb (CAB6089) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse brain using [KO Validated] HuR/ELAVL1 Rabbit pAb (CAB6089) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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