KO Validated Antibodies 1

Anti-HuR / ELAVL1 Antibody (CAB6089)[KO Validated]

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SKU:
CAB6089
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-HuR / ELAVL1 Antibody [KO Validated]
Antibody SKU:CAB6089
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HuR / ELAVL1 (NP_001410.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HeLa, MCF-7, Jurkat, 293T, Mouse spleen, Mouse thymus
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HuR / ELAVL1 (NP_001410.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSNG YEDH MAED CRGD IGRT NLIV NYLP QNMT QDEL RSLF SSIG EVES AKLI RDKV AGHS LGYG FVNY VTAK DAER AINT LNGL RLQS KTIK VSYA RPSS
Gene ID:1994
Uniprot:Q15717
Cellular Location:Cytoplasm, Nucleus
Calculated MW:36kDa/38kDa
Observed MW:36kDa
Synonyms:ELAV1, HUR, Hua, MelG, HuR / ELAVL1, ELAVL1
Background:The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy.
UniProt Protein Function:HuR: Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with ANP32A, EIF2C1 and EIF2C2. Ubiquitous. Belongs to the RRM elav family.
UniProt Protein Details:

Protein type:RNA-binding

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; membrane; cytoplasm; cytosol; nucleus

Molecular Function:mRNA binding; protein binding; mRNA 3'-UTR binding; RNA binding; double-stranded RNA binding; nucleotide binding; AU-rich element binding; protein kinase binding

Biological Process: positive regulation of translation; multicellular organismal development; mRNA stabilization; gene expression

NCBI Summary:The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy. [provided by RefSeq, Sep 2012]
UniProt Code:Q15717
NCBI GenInfo Identifier:20981691
NCBI Gene ID:1994
NCBI Accession:Q15717.2
UniProt Related Accession:Q15717
Molecular Weight:
NCBI Full Name:ELAV-like protein 1
NCBI Synonym Full Names:ELAV like RNA binding protein 1
NCBI Official Symbol:ELAVL1  
NCBI Official Synonym Symbols:HUR; Hua; MelG; ELAV1  
NCBI Protein Information:ELAV-like protein 1
UniProt Protein Name:ELAV-like protein 1
UniProt Synonym Protein Names:Hu-antigen R; HuR
UniProt Gene Name:ELAVL1  
UniProt Entry Name:ELAV1_HUMAN
Anti-HuR / ELAVL1 Antibody [KO Validated] (CAB6089)Western blot analysis of extracts of various cell lines, using HuR / ELAVL1 antibody (CAB6089) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
Anti-HuR / ELAVL1 Antibody [KO Validated] (CAB6089)Western blot analysis of extracts from normal (control) and HuR / ELAVL1 knockout (KO) 293T cells, using HuR / ELAVL1 antibody (CAB6089) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-HuR / ELAVL1 Antibody [KO Validated] (CAB6089)Immunohistochemistry of paraffin-embedded human esophagus using HuR / ELAVL1 antibody (CAB6089) at dilution of 1:100 (40x lens).
Anti-HuR / ELAVL1 Antibody [KO Validated] (CAB6089)Immunohistochemistry of paraffin-embedded human stomach using HuR / ELAVL1 antibody (CAB6089) at dilution of 1:100 (40x lens).
Anti-HuR / ELAVL1 Antibody [KO Validated] (CAB6089)Immunohistochemistry of paraffin-embedded mouse brain using HuR / ELAVL1 antibody (CAB6089) at dilution of 1:100 (40x lens).
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