Anti-Human CD166 (ALCAM) In Vivo Antibody - Low Endotoxin
- SKU:
- IVMB0248
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- 3A6
- Protein:
- CD166
- Isotype:
- IgG1 k
- Reactivity:
- Human
- Synonyms:
- CD6 ligand
- Synonyms:
- Activated Leukocyte Cell Adhesion Molecule (ALCAM)
- Research Area:
- Cell Adhesion
- Research Area:
- Cell Biology
- Research Area:
- Immunology
- Research Area:
- Neuroscience
- Research Area:
- Synaptic Biology
- Endotoxin Level:
- Low Endotoxin
- Host Species:
- Mouse
- Applications:
- FC
- Applications:
- IF
- Applications:
- IF Microscopy
- Applications:
- IHC
- Applications:
- In Vivo
- Applications:
- IP
Description
| Product Name: | Anti-Human CD166 (ALCAM) In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0248 |
| Size: | 1 mg, 5 mg, 25 mg, 50 mg, 100 mg |
| Clone: | 3A6 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | CD6 ligand, Activated Leukocyte Cell Adhesion Molecule (ALCAM) |
| Isotype: | IgG1 k |
| Reactivity: | Human |
| Applications: | FC, IF, IF Microscopy, IHC, In Vivo, IP |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC ⋅ >95% by SDS Page |
| Product Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Applications: | FC, IF, IF Microscopy, IHC, In Vivo, IP |
| Reactivity: | Human |
| Host Species: | Mouse |
| Specificity: | 3A6 activity is directed against human CD166 (ALCAM) and cross-reacts with ovine tissues. |
| Antigen Distribution: | CD166 is expressed on neurons, activated leukocytes, hematopoietic stem cells, mesenchymal stem cells, bone marrow stromal cells, activated T cells, activated B cells, activated monocytes, thymic epithelial cells, vascular endothelial cells, fibroblasts, keratinocytes, myeloid progenitors, tumor cells, and cancer stem cells. |
| Immunogen: | Cultured human thymic epithelial cells |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. |
| Purity: | ≥95% monomer by analytical SEC ⋅ >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles. |
Activated leukocyte cell adhesion molecule (ALCAM) is a member of the immunoglobulin superfamily and a cell surface glycoprotein1. In normal physiology, ALCAM functions in cell adhesion, is known to promote T cell activation and proliferation by interacting with CD6, and functions in angiogenesis, monocyte transmigration, leukocyte intravasation across the blood-brain barrier, hematopoiesis, neurite extension, osteogenesis, and embryonic implantation in the uterus. In cancer, ALCAM is a prognostic marker of disease progression and acts as a modulator of progression by controlling cell proliferation, adhesion, migration, and invasion.
ALCAM participates in homophilic ALCAM-ALCAM interactions as well as numerous heterophilic interactions1. Ligands include CD6, galectin-8, endophilin-A3/galectin-8, CD9, S100B, and ezrin. Additionally, SOSTDC1 is a novel ligand of ALCAM that promotes invasion and facilitates liver metastasis in colorectal cancer through activation of the Src-P13K/AKT pathways2.
ALCAM is a type I transmembrane molecule with a large glycosylated extracellular domain1. Two isoforms have been confirmed at the protein level: ALCAM-Iso1, which is the full length isoform, and ALCAM-Iso2, which lacks exon 13. ALCAM is proteolytically cleaved at its extracellular domain by the transmembrane metalloprotease ADAM17, with ALCAM-Iso2 more susceptible to cleavage.
3A6 was produced by immunizing mice with human thymic epithelial cells and then fusing spleen cells with P3X63Ag8 myeloma cells3. 3A6 cross reacts with ovine mesenchymal stromal cells from iliac crest bone marrow aspirates4.
| Research Area: | Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology |
| NCBI Gene Bank ID: | 214 |
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