Anti-Human CD166 (ALCAM) In Vivo Antibody - Low Endotoxin
Introducing the Anti-Human CD166/ALCAM In Vivo Antibody - Low Endotoxin from Assay Genie, a high-specificity monoclonal antibody tailored for in vivo applications. This antibody specifically targets the CD166/ALCAM protein, a critical molecule involved in cell adhesion processes, making it an excellent choice for research in oncology, immunology, and cell biology. With its mouse IgG1 isotype, it ensures high purity and minimal endotoxin levels (<1.0 EU/mg), ideal for a variety of assays including ELISA, flow cytometry, immunohistochemistry, and more. Offered in different sizes, this antibody is prepared in phosphate-buffered saline to maintain stability and effectiveness.
Elevate your research with confidence using this dependable and versatile antibody. CD166, also known as Activated Leukocyte Cell Adhesion Molecule (ALCAM), is expressed on the surface of activated T cells, monocytes, and various epithelial cells. It plays a vital role in mediating cell-cell interactions and is implicated in tumor progression and metastasis, underscoring its significance in cancer research and therapeutic development.
Product Name:
Anti-Human CD166 (ALCAM) In Vivo Antibody - Low Endotoxin
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
< 1.0 EU/mg as determined by the LAL method
Purity:
≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
FC, IF, IF Microscopy, IHC, In Vivo, IP
Reactivity:
Human
Host Species:
Mouse
Specificity:
3A6 activity is directed against human CD166 (ALCAM) and cross-reacts with ovine tissues.
Antigen Distribution:
CD166 is expressed on neurons, activated leukocytes, hematopoietic stem cells, mesenchymal stem cells, bone marrow stromal cells, activated T cells, activated B cells, activated monocytes, thymic epithelial cells, vascular endothelial cells, fibroblasts, keratinocytes, myeloid progenitors, tumor cells, and cancer stem cells.
Immunogen:
Cultured human thymic epithelial cells
Concentration:
≥ 5.0 mg/ml
Endotoxin Level:
< 1.0 EU/mg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.
Activated leukocyte cell adhesion molecule (ALCAM) is a member of the immunoglobulin superfamily and a cell surface glycoprotein1. In normal physiology, ALCAM functions in cell adhesion, is known to promote T cell activation and proliferation by interacting with CD6, and functions in angiogenesis, monocyte transmigration, leukocyte intravasation across the blood-brain barrier, hematopoiesis, neurite extension, osteogenesis, and embryonic implantation in the uterus. In cancer, ALCAM is a prognostic marker of disease progression and acts as a modulator of progression by controlling cell proliferation, adhesion, migration, and invasion.
ALCAM participates in homophilic ALCAM-ALCAM interactions as well as numerous heterophilic interactions1. Ligands include CD6, galectin-8, endophilin-A3/galectin-8, CD9, S100B, and ezrin. Additionally, SOSTDC1 is a novel ligand of ALCAM that promotes invasion and facilitates liver metastasis in colorectal cancer through activation of the Src-P13K/AKT pathways2.
ALCAM is a type I transmembrane molecule with a large glycosylated extracellular domain1. Two isoforms have been confirmed at the protein level: ALCAM-Iso1, which is the full length isoform, and ALCAM-Iso2, which lacks exon 13. ALCAM is proteolytically cleaved at its extracellular domain by the transmembrane metalloprotease ADAM17, with ALCAM-Iso2 more susceptible to cleavage.
3A6 was produced by immunizing mice with human thymic epithelial cells and then fusing spleen cells with P3X63Ag8 myeloma cells3. 3A6 cross reacts with ovine mesenchymal stromal cells from iliac crest bone marrow aspirates4.
