Name: Anti-HSPB1 Antibody (CAB16332)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB16332
Availability: OutOfStock

Antibody Name:	Anti-HSPB1 Antibody [KO Validated]
Antibody SKU:	CAB16332
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HSPB1 (NP_001531.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HSPB1 (NP_001531.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTER RVPF SLLR GPSW DPFR DWYP HSRL FDQA FGLP RLPE EWSQ WLGG SSWP GYVR PLPP AAIE SPAV AAPA YSRA LSRQ LSSG VSEI RHTA DRWR VSLD VNHF APDE LTVK TKDG VVEI
Gene ID:	3315
Uniprot:	P04792
Cellular Location:	Cytoplasm, Nucleus, cytoskeleton, spindle
Calculated MW:	22kDa
Observed MW:	27KDa

Synonyms:	HSPB1, CMT2F, HEL-S-102, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27
Background:	The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).

UniProt Protein Function:	HSP27: a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization. Its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.
UniProt Protein Details:	Protein type:Chaperone; Motility/polarity/chemotaxis; Heat shock protein Chromosomal Location of Human Ortholog: 7q11.23 Cellular Component: proteasome complex; extracellular space; focal adhesion; cytoskeleton; cytoplasm; plasma membrane; spindle; Z disc; nucleus; cytosol Molecular Function:identical protein binding; protein kinase C inhibitor activity; protein binding; ubiquitin binding; protein kinase C binding; protein kinase binding Biological Process: positive regulation of interleukin-1 beta production; positive regulation of tumor necrosis factor biosynthetic process; retinal homeostasis; positive regulation of angiogenesis; response to virus; positive regulation of blood vessel endothelial cell migration; negative regulation of protein kinase activity; gene expression; vascular endothelial growth factor receptor signaling pathway; cell motility; regulation of I-kappaB kinase/NF-kappaB cascade; response to unfolded protein; negative regulation of apoptosis; regulation of translational initiation Disease: Neuronopathy, Distal Hereditary Motor, Type Iib; Charcot-marie-tooth Disease, Axonal, Type 2f
NCBI Summary:	The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]
UniProt Code:	P04792
NCBI GenInfo Identifier:	19855073
NCBI Gene ID:	3315
NCBI Accession:	P04792.2
UniProt Secondary Accession:	P04792,Q6FI47, Q96C20, Q96EI7, Q9UC31, Q9UC34, Q9UC35 Q9UC36, B2R4N8,
UniProt Related Accession:	P04792
Molecular Weight:	205
NCBI Full Name:	Heat shock protein beta-1
NCBI Synonym Full Names:	heat shock 27kDa protein 1
NCBI Official Symbol:	HSPB1
NCBI Official Synonym Symbols:	CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
NCBI Protein Information:	heat shock protein beta-1; HSP 27; 28 kDa heat shock protein; heat shock 27 kDa protein; heat shock 27kD protein 1; stress-responsive protein 27; estrogen-regulated 24 kDa protein; epididymis secretory protein Li 102
UniProt Protein Name:	Heat shock protein beta-1
UniProt Synonym Protein Names:	28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27
Protein Family:	Heat shock protein
UniProt Gene Name:	HSPB1
UniProt Entry Name:	HSPB1_HUMAN

	Western blot analysis of extracts from normal (control) and HSPB1 knockout (KO) HeLa cells, using HSPB1 antibody (CAB16332) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Western blot analysis of extracts of HeLa cells, using HSPB1 antibody (CAB16332) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human breast cancer using HSPB1 antibody (CAB16332) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat ovary using HSPB1 antibody (CAB16332) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using HSPB1 antibody (CAB16332) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-HSPB1 Antibody (CAB0240)	Human HSP-27/HSPB1 (Heat Shock Protein 27) CLIA Kit (HUES01027)
Anti-HSPB1 Mouse Monoclonal Antibody (CAB10606)	Human Heat shock protein beta-1 (HSPB1) ELISA Kit
Anti-HSPB1 Antibody (CAB13681)	HSP27 (Phospho-Ser82) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-HSPB1-S15 Antibody (CABP0040)	HSP27 Colorimetric Cell-Based ELISA Kit
Anti-Phospho-HSPB1-S82 Antibody (CABP0041)	HSP27 (Phospho-Ser15) Colorimetric Cell-Based ELISA Kit