The HOXB4 Monoclonal Antibody (CAB19699) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it an ideal choice for various research applications, including Western blotting.HOXB4 is known for its ability to promote the expansion of hematopoietic stem cells, making it a promising target for therapeutic interventions in conditions like hematologic malignancies and bone marrow failure syndromes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
HOXB4 Monoclonal Antibody
SKU:
CAB19699
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC2236
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HOX2, HOX2F, HOX-2.6, HOXB4
Positive Sample:
293T, K-562
Cellular Localization:
Centrosome, Nucleoplasm, Nucleus.
Calculated MW:
28kDa
Observed MW:
34kDa
This gene is a member of the Antp homeobox family and encodes a nuclear protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in development. Intracellular or ectopic expression of this protein expands hematopoietic stem and progenitor cells in vivo and in vitro, making it a potential candidate for therapeutic stem cell expansion.
Purification Method
Affinity purification
Gene ID
3214
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HOXB4 Rabbit mAb (CAB19699) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
