Cell Biology Antibodies 6

HOOK3 Rabbit Polyclonal Antibody (CAB15536)

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SKU:
CAB15536
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
HK3, HOOK3
Research Use:
Cell Biology
Frequently bought together:

Description

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HOOK3 Antibody (CAB15536)

The HOOK3 Antibody (CAB15536) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for Western blot analysis.HOOK3 is involved in various cellular processes, including intracellular transport, ciliary biogenesis, and cell division. Its dysfunction has been linked to developmental disorders and neurodegenerative diseases, making it a crucial target for investigation in the fields of cell biology and neuroscience.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:HOOK3 Antibody
SKU:CAB15536
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:IALL QSLL DDAN LRKN ELET ENRL VNQR LLEV QSQV EELQ KSLQ DQGS KAED SVLL KKKL EEHL EKLH EANN ELQK KRAI IEDL EPRF NNSS LKIE ELQE ALRK KEEE MKQM EERY KKYL EKAK SVIR TLDP KQNQ GAAP EIQA LKNQ LQER DRLF HSLE KEYE KTKS QREM EEKY IVSA WYNM GMTL HKKA AEDR LAST GSGQ SFLA RQRQ ATSS RRSY PGHV QPAT AR
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:200 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HK3, HOOK3
Positive Sample:LO2, HeLa, 293T, mouse heart, mouse kidney, rat lung
Cellular Localization:Cytoplasm, Golgi Apparatus, Cytoskeleton.
Calculated MW:83kDa
Observed MW:170kDa

Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.

Purification MethodAffinity purification
Gene ID84376
RRIDAB_2762937
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using HOOK3 Rabbit pAb (CAB15536) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunofluorescence analysis of C6 cells using HOOK3 Rabbit pAb (CAB15536) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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