Cell Death Antibodies 1

Heme Oxygenase 1 (HO-1/HMOX1) Rabbit Polyclonal Antibody (CAB1346)

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SKU:
CAB1346
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Reactivity:
Human, Mouse, Rat
Synonyms:
HO-1, HSP32, HMOX1D, bK286B10, Heme Oxygenase 1 (HO-1/HMOX1)
Research Use:
Cell Death
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Description

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Heme Oxygenase 1 (HO-1/HMOX1) Antibody (CAB1346)

The Heme Oxygenase 1 (HO-1/HMOX1) Antibody (CAB1346) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to heme oxygenase-1, researchers can detect and analyze this enzyme in a variety of cell types, making it an ideal choice for studies in oxidative stress, inflammation, and diseases such as cardiovascular disease and cancer.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Heme Oxygenase 1 (HO-1/HMOX1) Antibody
SKU:CAB1346
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MERP QPDS MPQD LSEA LKEA TKEV HTQA ENAE FMRN FQKG QVTR DGFK LVMA SLYH IYVA LEEE IERN KESP VFAP VYFP EELH RKAA LEQD LAFW YGPR WQEV IPYT PAMQ RYVK RLHE VGRT EPEL LVAH AYTR YLGD LSGG QVLK KIAQ KALD LPSS GEGL AFFT FPNI ASAT KFKQ LYRS RMNS LEMT PAVR QRVI EEAK TAFL LNIQ LFEE LQEL LTHD TKDQ SPSR APGL RQRA SNKV QDSA PVET PRGK PPLN TRSQ APLL RWVL TLSF LVAT VAVG LYAM
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:1000 - 1:5000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HO-1, HSP32, HMOX1D, bK286B10, Heme Oxygenase 1 (HO-1/HMOX1)
Positive Sample:A549, NIH/3T3, Hep G2, , HeLa
Cellular Localization:Cytoplasmic Side, Endoplasmic Reticulum Membrane, Microsome, Peripheral Membrane Protein.
Calculated MW:33kDa
Observed MW:35kDa

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.

Purification MethodAffinity purification
Gene ID3162
RRIDAB_2760320
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from A549 cells, using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (CAB1346) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageWestern blot analysis of lysates from NIH/3T3 cells using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (CAB1346) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human liver using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (CAB1346) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of HeLa cells using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (CAB1346) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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