Cell Death Antibodies 1

Heme Oxygenase 1 (HO-1/HMOX1) Rabbit Polyclonal Antibody (CAB11102)

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SKU:
CAB11102
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Reactivity:
Human, Mouse, Rat
Synonyms:
HO-1, HSP32, HMOX1D, bK286B10, Heme Oxygenase 1 (HO-1/HMOX1)
Research Use:
Cell Death
Frequently bought together:

Description

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Heme Oxygenase 1 (HO-1/HMOX1) Antibody (CAB11102)

The Heme Oxygenase 1 (HO-1/HMOX1) Antibody (CAB11102) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly specific for human samples and has been validated for use in Western blot applications. By binding to the HO-1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it a valuable asset for investigations in oxidative stress, inflammation, and diseases like cancer and cardiovascular disorders.HO-1, a stress-responsive enzyme, plays a crucial role in the breakdown of heme into biliverdin, carbon monoxide, and iron, leading to the antioxidant and cytoprotective effects that are essential for cellular survival under oxidative conditions.

This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Heme Oxygenase 1 (HO-1/HMOX1) Antibody
SKU:CAB11102
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MERP QPDS MPQD LSEA LKEA TKEV HTQA ENAE FMRN FQKG QVTR DGFK LVMA SLYH IYVA LEEE IERN KESP VFAP VYFP EELH RKAA LEQD LAFW YGPR WQEV IPYT PAMQ RYVK RLHE VGRT EPEL LVAH AYTR YLGD LSGG QVLK KIAQ KALD LPSS GEGL AFFT FPNI ASAT KFKQ LYRS RMNS LEMT PAVR QRVI EEAK TAFL LNIQ LFEE LQEL LTHD TKDQ SPSR APGL RQRA SNKV QDSA PVET PRGK PPLN TRSQ A
Tested Applications:WB IHC-P ELISA IF-P
Recommended Dilution:
WB1:500 - 1:1000
IF-P1:100 - 1:200
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HO-1, HSP32, HMOX1D, bK286B10, Heme Oxygenase 1 (HO-1/HMOX1)
Positive Sample:Mouse spleen, Hep G2, A549
Cellular Localization:Cytoplasmic Side, Endoplasmic Reticulum Membrane, Microsome, Peripheral Membrane Protein.
Calculated MW:33kDa
Observed MW:30kDa

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.

Purification MethodAffinity purification
Gene ID3162
RRIDAB_2758406
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Mouse spleen, using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (CAB11102) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Product ImageWestern blot analysis of various lysates using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (CAB11102) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat spleen using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (CAB11102) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageConfocal imaging of paraffin-embedded Mouse spleen tissue using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (CAB11102, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
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