Epigenetics & Nuclear Signaling Antibodies 3

HNRNPF Rabbit Polyclonal Antibody (CAB5505)

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SKU:
CAB5505
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse
Synonyms:
HNRPF, mcs94-1, OK/SW-cl.23, PF
Research Use:
Epigenetics and Nuclear Signaling
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Description

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HNRNPF Antibody (CAB5505)

The HNRNPF Antibody (CAB5505) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the HNRNPF protein, researchers can accurately analyze its expression levels in various cell types, making it an excellent choice for studies in molecular biology and genomics.HNRNPF is a multi-functional protein with diverse roles in RNA splicing, transport, and translation.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:HNRNPF Antibody
SKU:CAB5505
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MMLG PEGG EGFV VKLR GLPW SCSV EDVQ NFLS DCTI HDGA AGVH FIYT REGR QSGE AFVE LGSE DDVK MALK KDRE SMGH RYIE VFKS HRTE MDWV LKHS GPNS ADSA NDGF VRLR GLPF GCTK EEIV QFFS GLEI VPNG ITLP VDPE GKIT GEAF VQFA SQEL AEKA LGKH KERI GHRY IEVF KSSQ EEVR SYSD PPLK FMSV QRPG PYDR PGTA RRYI GIVK QAGL ERMR PGAY STGY GGYE EYSG LSDG YGFT TDLF GRDL SYCL SGMY DHRY GDSE
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:1000 - 1:5000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HNRPF, mcs94-1, OK/SW-cl.23, PF
Positive Sample:22Rv1, Lovo, MCF7, BT-474, Mouse spleen
Cellular Localization:Nucleus, Nucleoplasm.
Calculated MW:46kDa
Observed MW:47kDa

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Purification MethodAffinity purification
Gene ID3185
RRIDAB_2766300
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] HNRNPF Rabbit pAb (CAB5505) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageWestern blot analysis of lysates from wild type(WT) and HNRNPF knockout (KO) 293T cells, using [KO Validated] HNRNPF Rabbit pAb (CAB5505) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
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