Cell Biology Antibodies 11

HMGCL Rabbit Polyclonal Antibody (CAB8052)

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SKU:
CAB8052
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
HL, HMGCL
Research Use:
Cell Biology
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Description

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HMGCL Antibody (CAB8052)

The HMGCL Antibody (CAB8052) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot analyses.HMGCL plays a crucial role in breaking down fats for energy production, making it a key target for studying metabolic disorders like ketosis and hypercholesterolemia. By binding to the HMGCL protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for research in metabolism, lipid metabolism disorders, and mitochondrial diseases.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:HMGCL Antibody
SKU:CAB8052
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MAAM RKAL PRRL VGLA SLRA VSTS SMGT LPKR VKIV EVGP RDGL QNEK NIVS TPVK IKLI DMLS EAGL SVIE TTSF VSPK WVPQ MGDH TEVL KGIQ KFPG INYP VLTP NLKG FEAA VAAG AKEV VIFG AASE LFTK KNIN CSIE ESFQ RFDA ILKA AQSA NISV RGYV SCAL GCPY EGKI SPAK VAEV TKKF YSMG CYEI SLGD TIGV GTPG IMKD MLSA VMQE VPLA ALAV HCHD TYGQ ALAN TLMA LQMG VSVV DSSV AGLG GCPY AQGA SGNL ATED LVYM LEGL GIHT GVNL QKLL EAGN FICQ ALNR KTSS KVAQ ATCK L
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HL, HMGCL
Positive Sample:U-251MG, SKOV3, BT-474, MCF7, Mouse liver, Mouse kidney
Cellular Localization:Mitochondrion Matrix, Peroxisome.
Calculated MW:34kDa
Observed MW:30kDa

The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID3155
RRIDAB_2769810
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using HMGCL Rabbit pAb (CAB8052) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 15s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat kidney using HMGCL Rabbit pAb (CAB8052) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human kidney using HMGCL Rabbit pAb (CAB8052) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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