KO Validated Antibodies 1

Anti-HK1 Antibody (CAB1054)[KO Validated]

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SKU:
CAB1054
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-HK1 Antibody [KO Validated]
Antibody SKU:CAB1054
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human HK1 (NP_000179.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, MCF7, U-87MG, Mouse testis, Mouse brain, Mouse heart, Rat testis, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human HK1 (NP_000179.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KKID KYLY AMRL SDET LIDI MTRF RKEM KNGL SRDF NPTA TVKM LPTF VRSI PDGS EKGD FIAL DLGG SSFR ILRV QVNH EKNQ NVHM ESEV YDTP ENIV HGSG SQLF DHVA ECLG DFME KRKI KDKK LPVG FTFS FPCQ QSKI DEAI LITW TKRF KASG VEGA DVVK LLNK AIKK RGDY DANI VAVV NDTV GTMM TCGY DDQH CEVG LIIG TGTN ACYM EELR HIDL VEGD EGRM CINT EWGA FGDD GSLE DIRT EFDR EIDR GSLN PGKQ LFEK MVSG M
Gene ID:3098
Uniprot:P19367
Cellular Location:Mitochondrion outer membrane
Calculated MW:101kDa/102kDa
Observed MW:102KDa
Synonyms:HK1, HK, HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, hexokinase, RP79
Background:Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.
NCBI Summary:Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016]
UniProt Code:P19367
NCBI GenInfo Identifier:116242516
NCBI Gene ID:3098
NCBI Accession:P19367.3
UniProt Secondary Accession:P19367,O43443, O43444, O75574, Q5VTC3, Q96HC8, Q9NNZ4 Q9NNZ5, E9PCK0,
UniProt Related Accession:P19367
Molecular Weight:101,085 Da
NCBI Full Name:Hexokinase-1
NCBI Synonym Full Names:hexokinase 1
NCBI Official Symbol:HK1  
NCBI Official Synonym Symbols:HK; HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc; hexokinase  
NCBI Protein Information:hexokinase-1
UniProt Protein Name:Hexokinase-1
UniProt Synonym Protein Names:Brain form hexokinase; Hexokinase type I; HK I
Protein Family:Probable histidine kinase
UniProt Gene Name:HK1  
UniProt Entry Name:HXK1_HUMAN
Anti-HK1 Antibody [KO Validated] (CAB1054)Western blot analysis of extracts of various cell lines, using HK1 antibody (CAB1054) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-HK1 Antibody [KO Validated] (CAB1054)Western blot analysis of extracts from normal (control) and HK1 knockout (KO) 293T cells, using HK1 antibody (CAB1054) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-HK1 Antibody [KO Validated] (CAB1054)Immunohistochemistry of paraffin-embedded rat kidney using HK1 Rabbit pAb (CAB1054) at dilution of 1:150 (40x lens).
Anti-HK1 Antibody [KO Validated] (CAB1054)Immunohistochemistry of paraffin-embedded mouse kidney using HK1 Rabbit pAb (CAB1054) at dilution of 1:150 (40x lens).
Anti-HK1 Antibody [KO Validated] (CAB1054)Immunofluorescence analysis of U2OS cells using HK1 Rabbit pAb (CAB1054) at dilution of 1:100. Blue: DAPI for nuclear staining.
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