Cell Biology Antibodies 5

HIGD1A Rabbit Polyclonal Antibody (CAB14582)

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SKU:
CAB14582
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Synonyms:
HIG1, RCF1a, HIGD1A
Research Use:
Cell Biology
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Description

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HIGD1A Antibody (CAB14582)

The HIGD1A Antibody (CAB14582) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the HIGD1A protein, this antibody allows for the detection and analysis of HIGD1A in various cell types, making it ideal for studies in cell biology and cancer research.HIGD1A, also known as hypoxia-inducible gene domain family member 1A, plays a crucial role in maintaining cellular homeostasis under conditions of low oxygen levels.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:HIGD1A Antibody
SKU:CAB14582
Size:20μL, 100μL
Reactivity:Human
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MSTD TGVS LPSY EEDQ GSKL IRKA KEAP FVPV GIAG FAAI VAYG LYKL KSRG NTKM SIHL IHMR VAAQ GFVV GAMT VGMG YSMY REFW AKPK P
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:100
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HIG1, RCF1a, HIGD1A
Positive Sample:HeLa, HepG2, 293T, K-562
Cellular Localization:Mitochondrion Inner Membrane, Mitochondrion Membrane, Multi-Pass Membrane Protein.
Calculated MW:10kDa
Observed MW:14kDa

Acts upstream of or within negative regulation of apoptotic process. Located in mitochondrion and nucleoplasm. Part of protein-containing complex.

Purification MethodAffinity purification
Gene ID25994
RRIDAB_2761456
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using HIGD1A Rabbit pAb (CAB14582) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon carcinoma using HIGD1A Rabbit pAb (CAB14582) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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