KO Validated Antibodies 1

[KO Validated] HIF1AN/FIH1 Rabbit Polyclonal Antibody (CAB5466)

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SKU:
CAB5466
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
FIH1, H1
Research Use:
Epigenetics and Nuclear Signaling
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Description

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[KO Validated] HIF1AN/FIH1 Antibody (CAB5466)

The [KO Validated] HIF1AN/FIH1 Antibody (CAB5466) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is specifically designed for use in detecting and analyzing HIF1AN in human samples. Validated for Western blot applications, it provides researchers with a reliable means of studying the expression and function of HIF1AN in different cell types.HIF1AN, also known as FIH-1, acts as a negative regulator of the hypoxia inducible factor (HIF) pathway, influencing various cellular processes such as metabolism, angiogenesis, and apoptosis.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] HIF1AN/FIH1 Antibody
SKU:CAB5466
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MAAT AAEA VASG SGEP REEA GALG PAWD ESQL RSYS FPTR PIPR LSQS DPRA EELI ENEE PVVL TDTN LVYP ALKW DLEY LQEN IGNG DFSV YSAS THKF LYYD EKKM ANFQ NFKP RSNR EEMK FHEF VEKL QDIQ QRGG EERL YLQQ TLND TVGR KIVM DFLG FNWN WINK QQGK RGWG QLTS NLLL IGME GNVT PAHY DEQQ NFFA QIKG YKRC ILFP PDQF ECLY PYPV HHPC DRQS QVDF DNPD YERF PNFQ NVVG YETV VGPG DVLY IPMY WWHH IESL LNGG ITIT VNFW YKGA PTPK RIEY PLKA HQKV AIMR NIEK MLGE ALGN PQEV GPLL NTMI KGRY N
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:FIH1, H1
Positive Sample:MCF7, K-562, U-937, 293T, Mouse heart, Mouse skeletal muscle
Cellular Localization:Cytoplasm, Nucleus, Perinuclear Region.
Calculated MW:41kDa
Observed MW:40kDa

Enables several functions, including 2-oxoglutarate-dependent dioxygenase activity; NF-kappaB binding activity; and transition metal ion binding activity. Involved in several processes, including negative regulation of Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter in response to hypoxia; and protein hydroxylation. Located in cytosol; nucleoplasm; and perinuclear region of cytoplasm. Colocalizes with nucleus.

Purification MethodAffinity purification
Gene ID55662
RRIDAB_2766267
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] HIF1AN/FIH1 Rabbit pAb (CAB5466) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageWestern blot analysis of lysates from wild type (WT) and HIF1AN/FIH1 knockout (KO) 293T cells, using [KO Validated] HIF1AN/FIH1 Rabbit pAb (CAB5466) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using [KO Validated] HIF1AN/FIH1 Rabbit pAb (CAB5466) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat kidney tissue using [KO Validated] HIF1AN/FIH1 Rabbit pAb (CAB5466) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunoprecipitation analysis of 200 μg extracts of 293T cells using 1 μg HIF1AN/FIH1 antibody (CAB5466). Western blot was performed from the immunoprecipitate using HIF1AN/FIH1 antibody (CAB5466) at a dilution of 1:1000.
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