Epigenetics & Nuclear Signaling Antibodies 1

HDGF Rabbit Polyclonal Antibody (CAB13654)

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SKU:
CAB13654
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Synonyms:
HMG1L2, HDGF
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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HDGF Antibody (CAB13654)

The HDGF Antibody (CAB13654) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high reactivity with human samples and is suitable for use in Western blot applications. By specifically binding to the HDGF protein, this antibody enables the detection and analysis of HDGF expression in a variety of cell types.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:HDGF Antibody
SKU:CAB13654
Size:20μL, 100μL
Reactivity:Human
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MSRS NRQK EYKC GDLV FAKM KGYP HWPA RIDE MPEA AVKS TANK YQVF FFGT HETA FLGP KDLF PYEE SKEK FGKP NKRK GFSE GLWE IENN PTVK ASGY QSSQ KKSC VEEP EPEP EAAE GDGD KKGN AEGS SDEE GKLV IDEP AKEK NEKG ALKR RAGD LLED SPKR PKEA ENPE GEEK EAAT LEVE RPLP MEVE KNST PSEP GSGR GPPQ EEEE EEDE EEEA TKED AEAP GIRD HESL
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HMG1L2, HDGF
Positive Sample:22Rv1, U-251MG, MCF7
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:27kDa
Observed MW:37kDa

This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Purification MethodAffinity purification
Gene ID3068
RRIDAB_2760516
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using HDGF Rabbit pAb (CAB13654) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human lung cancer using HDGF Rabbit pAb (CAB13654) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human breast cancer using HDGF Rabbit pAb (CAB13654) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
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