Cell Biology Antibodies 5

GOLGA1 Rabbit Polyclonal Antibody (CAB14688)

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SKU:
CAB14688
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
golgin-97, GOLGA1
Research Use:
Cell Biology
Frequently bought together:

Description

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GOLGA1 Antibody (CAB14688)

The GOLGA1 Antibody (CAB14688) is a high-quality antibody developed for reliable detection and analysis of target proteins. Golga1, also known as Golgin-97, is a Golgi matrix protein involved in maintaining Golgi structure and mediating protein trafficking within the cell.This antibody, produced in rabbits, is highly specific for Golga1 in various cell types and species, making it an excellent choice for immunofluorescence, immunohistochemistry, and Western blot applications. By detecting Golga1 protein expression and localization, researchers can gain insights into Golgi organization and its role in protein sorting and secretion pathways.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:GOLGA1 Antibody
SKU:CAB14688
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MFAK LKKK IAEE TAVA QRPG GATR IPRS VSKE SVAS MGAD SGDD FASD GSSS REDL SSQL LRRN EQIR KLEA RLSD YAEQ VRNL QKIK EKLE IALE KHQD SSMR KFQE QNET FQAN RAKM AEGL ALAL ARKD QEWS EKMD QLEK EKNI LTAQ LQEM KNQS MNLF QRRD EMDE LEGF QQQE LSKI KHML LKKE ESLG KMEQ ELEA RTRE LSRT QEEL MNSN QMSS DLSQ KLEE LQRH YSTL EEQR DHVI ASKT GAES KITA LEQK EQEL QALI QQLS IDLQ KVTA ETQE KEDV ITHL QEKV
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:golgin-97, GOLGA1
Positive Sample:HepG2, HeLa, SW480, Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat pancreas
Cellular Localization:Golgi Apparatus, Golgi Stack Membrane, Peripheral Membrane Protein.
Calculated MW:88kDa
Observed MW:105kDa

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein is associated with Sjogren's syndrome.

Purification MethodAffinity purification
Gene ID2800
RRIDAB_2761563
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using GOLGA1 Rabbit pAb (CAB14688) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Product ImageImmunofluorescence analysis of HepG2 cells using GOLGA1 Rabbit pAb (CAB14688) at dilution of 1:150. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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