Cell Biology Antibodies 9

GNRH1 Rabbit Polyclonal Antibody (CAB5625)

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SKU:
CAB5625
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
GRH, GNRH, LHRH, LNRH, GNRH1
Research Use:
Cell Biology
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Description

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GNRH1 Antibody (CAB5625)

The GNRH1 Antibody (CAB5625) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in immunohistochemistry and ELISA applications. By binding to Progonadoliberin-1, researchers can accurately detect and analyze the protein in various tissues and cell types, making it an essential component for studies in reproductive biology and hormone regulation.Progonadoliberin-1, also known as luteinizing hormone-releasing hormone, plays a pivotal role in stimulating the release of reproductive hormones from the pituitary gland, which in turn regulates the menstrual cycle, ovulation, and fertility.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:GNRH1 Antibody
SKU:CAB5625
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:QHWS YGLR PGGK RDAE NLID SFQE IVKE VGQL AETQ RFEC TTHQ PRSP LRDL KGAL ESLI EEET GQKK I
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:GRH, GNRH, LHRH, LNRH, GNRH1
Positive Sample:Mouse lung, Rat kidney
Cellular Localization:Secreted.
Calculated MW:10kDa
Observed MW:12kDa

This gene encodes a preproprotein that is proteolytically processed to generate a peptide that is a member of the gonadotropin-releasing hormone (GnRH) family of peptides. Alternative splicing results in multiple transcript variants, at least one of which is secreted and then cleaved to generate gonadoliberin-1 and GnRH-associated peptide 1. Gonadoliberin-1 stimulates the release of luteinizing and follicle stimulating hormones, which are important for reproduction. Mutations in this gene are associated with hypogonadotropic hypogonadism.

Purification MethodAffinity purification
Gene ID2796
RRIDAB_2766385
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Mouse lung, using GNRH1 Rabbit pAb (CAB5625) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageWestern blot analysis of lysates from Rat kidney, using GNRH1 Rabbit pAb (CAB5625) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using GNRH1 Rabbit pAb (CAB5625) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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