KO Validated Antibodies 1

[KO Validated] GNAI3 Rabbit Polyclonal Antibody (CAB13307)

(No reviews yet) Write a Review
SKU:
CAB13307
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
Reactivity:
Human, Mouse
Synonyms:
87U6, HG1A, ARCND1, ARCODS, I3
Research Use:
Cell Cycle
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

[KO Validated] GNAI3 Antibody (CAB13307)

The [KO Validated] GNAI3 Antibody (CAB13307) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high specificity and sensitivity towards human samples, making it a reliable tool for Western blot applications. By binding specifically to GNAI3, this antibody enables the detection and analysis of the protein in various cell types, making it an essential component for studies in cell signaling and cancer research.GNAI3, a member of the G protein family, plays a crucial role in mediating intracellular signaling cascades triggered by various extracellular stimuli.

This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:[KO Validated] GNAI3 Antibody
SKU:CAB13307
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MGCT LSAE DKAA VERS KMID RNLR EDGE KAAK EVKL LLLG AGES GKST IVKQ MKII HEDG YSED ECKQ YKVV VYSN TIQS IIAI IRAM GRLK IDFG EAAR ADDA RQLF VLAG SAEE GVMT PELA GVIK RLWR DGGV QACF SRSR EYQL NDSA SYYL NDLD RISQ SNYI PTQQ DVLR TRVK TTGI VETH FTFK DLYF KMFD VGGQ RSER KKWI HCFE GVTA IIFC VALS DYDL VLAE DEEM NRMH ESMK LFDS ICNN KWFT ETSI ILFL NKKD LFEE KIKR SPLT ICYP EYTG SNTY EEAA AYIQ CQFE DLNR RKDT KEIY THFT CATD TKNV QFVF DAVT DVII KNNL KECG LY
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:87U6, HG1A, ARCND1, ARCODS, I3
Positive Sample:LO2, MCF-7, HL-60, HT-1080
Cellular Localization:Cell Membrane, Cytoplasm, Lipid-Anchor, Membrane, Centrosome, Cytoskeleton, Microtubule Organizing Center.
Calculated MW:41kDa
Observed MW:45kDa

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway.

Purification MethodAffinity purification
Gene ID2773
RRIDAB_2861683
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] GNAI3 Rabbit pAb (CAB13307) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageWestern blot analysis of lysates from wild type (WT) and GNAI3 knockout (KO) HeLa cells, using [KO Validated] GNAI3 Rabbit pAb (CAB13307) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunoprecipitation analysis of 200 μg extracts of MCF-7 cells, using 3 μg GNAI3 antibody (CAB13307). Western blot was performed from the immunoprecipitate using GNAI3 antibody (CAB13307) at a dilution of 1:1000.
View AllClose

0 Reviews

View AllClose

Related Products