Antibody Name:	Anti-GLUL Antibody [KO Validated]
Antibody SKU:	CAB5437
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-373 of human GLUL (NP_001028228.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	K-562, U-87MG, Mouse intestine, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-373 of human GLUL (NP_001028228.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTTS ASSH LNKG IKQV YMSL PQGE KVQA MYIW IDGT GEGL RCKT RTLD SEPK CVEE LPEW NFDG SSTL QSEG SNSD MYLV PAAM FRDP FRKD PNKL VLCE VFKY NRRP AETN LRHT CKRI MDMV SNQH PWFG MEQE YTLM GTDG HPFG WPSN GFPG PQGP YYCG VGAD RAYG RDIV EAHY RACL YAGV KIAG TNAE VMPA QWEF QIGP CEGI SMGD HLWV ARFI LHRV CEDF GVIA TFDP KPIP GNWN GAGC HTNF STKA MREE NGLK YIEE AIEK LSKR HQYH IRAY DPKG GLDN ARRL TGFH ETSN INDF SAGV ANRS ASIR IPRT VGQE KKGY FEDR RPSA NCDP FSVT EALI RTCL LNET GDEP FQYK N
Gene ID:	2752
Uniprot:	P15104
Cellular Location:	Cytoplasm, Mitochondrion
Calculated MW:	42kDa
Observed MW:	40kDa

Synonyms:

GLNS, GS, PIG43, PIG59, GLUL

Background:

The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	GLUL: This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner. Essential for proliferation of fetal skin fibroblasts. Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD). CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid. Belongs to the glutamine synthetase family.
UniProt Protein Details:	Protein type:Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; EC 6.3.1.2; Energy Metabolism - nitrogen; EC 4.1.1.15; Ligase Chromosomal Location of Human Ortholog: 1q31 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:glutamate-ammonia ligase activity; identical protein binding; protein binding Biological Process: amino acid biosynthetic process; cell proliferation; glutamate catabolic process; glutamine biosynthetic process; neurotransmitter uptake Disease: Glutamine Deficiency, Congenital
NCBI Summary:	The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
UniProt Code:	P15104
NCBI GenInfo Identifier:	1169929
NCBI Gene ID:	2752
NCBI Accession:	P15104.4
UniProt Secondary Accession:	P15104,Q499Y9, Q5T9Z1, Q7Z3W4, Q8IZ17,
UniProt Related Accession:	P15104
Molecular Weight:	42,064 Da
NCBI Full Name:	Glutamine synthetase
NCBI Synonym Full Names:	glutamate-ammonia ligase
NCBI Official Symbol:	GLUL
NCBI Official Synonym Symbols:	GS; GLNS; PIG43; PIG59
NCBI Protein Information:	glutamine synthetase
UniProt Protein Name:	Glutamine synthetase
UniProt Synonym Protein Names:	Glutamate decarboxylase (EC:4.1.1.15); Glutamate--ammonia ligase
UniProt Gene Name:	GLUL
UniProt Entry Name:	GLNA_HUMAN

	Western blot analysis of extracts of various cell lines, using GLUL antibody (CAB5437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and GLUL knockout (KO) HeLa cells, using GLUL antibody (CAB5437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunohistochemistry of paraffin-embedded human stomach using GLUL Antibody (CAB5437) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using GLUL Antibody (CAB5437) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse stomach using GLUL Antibody (CAB5437) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-Glutamine Synthetase Antibody (CAB19641)	Human GLN1 / Glutamine Synthetase ELISA Kit
GLUL Antibody (PACO01754)	Mouse GS (Gelsolin) CLIA Kit (MOES00287)
GLUL Antibody (PACO09493)	Human Glutamine synthetase (GLUL) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)