Epigenetics & Nuclear Signaling Antibodies 1

GATA4 Rabbit Polyclonal Antibody (CAB13756)

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SKU:
CAB13756
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
TOF, ASD2, VSD1, TACHD, GATA4
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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GATA4 Antibody (CAB13756)

The GATA4 Antibody (CAB13756) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high specificity and sensitivity for detecting GATA4 in human samples, making it ideal for use in Western blot applications. By targeting the GATA4 protein, this antibody allows for accurate and reliable analysis of its expression levels in various cell types, offering insight into its role in cardiac biology and disease.GATA4 is essential for the proper formation and functioning of the heart, making it a critical target for investigation in cardiovascular research.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:GATA4 Antibody
SKU:CAB13756
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:PPAS GASS NSSN ATTS SSEE MRPI KTEP GLSS HYGH SSSV SQTF SVSA MSGH GPSI HPVL SALK LSPQ GYAS PVSQ SPQT SSKQ DSWN SLVL ADSH GDII
Tested Applications:WB IHC-P IF/ICC ELISA IF-P
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
IF-P1:50 - 1:200
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:TOF, ASD2, VSD1, TACHD, GATA4
Positive Sample:SKOV3, Mouse testis, Rat stomach
Cellular Localization:Nucleus.
Calculated MW:45kDa
Observed MW:50kDa

This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID2626
RRIDAB_2760613
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using GATA4 Rabbit pAb (CAB13756) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageWestern blot analysis of lysates from Rat stomach, using GATA4 Rabbit pAb (CAB13756) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse lung using GATA4 Rabbit pAb (CAB13756) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat spleen using GATA4 Rabbit pAb (CAB13756) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageConfocal imaging of paraffin-embedded Human testis tissue using GATA4 Rabbit pAb (CAB13756, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Product ImageConfocal imaging of paraffin-embedded Mouse testis tissue using GATA4 Rabbit pAb (CAB13756, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
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