Description
Antibody Name: | Anti-GALT Antibody (CAB20966) |
Antibody SKU: | CAB20966 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting |
Reactivity: | Human |
Host Species: | Rabbit |
Immunogen: | Recombinant protein of Human GALT. |
Application: | Western blotting |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Human |
Positive Samples: | HeLa, U-251MG |
Immunogen: | Recombinant protein of Human GALT. |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Cellular Location: | |
Calculated MW: | 31kDa/43kDa |
Observed MW: | 43KDa |
Synonyms: | GALT |
Background: | Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene. |
Western blot analysis of extracts of various cell lines, using GALT antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |