Epigenetics & Nuclear Signaling Antibodies 2

FUBP1 Rabbit Polyclonal Antibody (CAB5587)

(No reviews yet) Write a Review
SKU:
CAB5587
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
FBP, FUBP, hDH V, FUBP1
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

FUBP1 Antibody (CAB5587)

The FUBP1 Antibody (CAB5587) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blotting applications.FUBP1, also known as far upstream element-binding protein 1, plays a key role in controlling the expression of genes involved in cell growth, differentiation, and survival. Dysregulation of FUBP1 has been implicated in various diseases, including cancer, making it an attractive target for research aimed at understanding the molecular mechanisms underlying these conditions.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:FUBP1 Antibody
SKU:CAB5587
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:QNDA GVRI QFKP DDGT TPER IAQI TGPP DRCQ HAAE IITD LLRS VQAG NPGG PGPG GRGR GRGQ GNWN MGPP GGLQ EFNF IVPT GKTG LIIG KGGE TIKS ISQQ SGAR IELQ RNPP PNAD PNMK LFTI RGTP QQID YARQ LIEE KIGG PVNP LGPP VPHG PHGV PGPH GPPG PPGP GTPM GPYN PAPY NPGP PGPA PHGP PAPY APQG WGNA YPHW QQQA PPDP AKAG TDPN SAAW AAYY AHYY QQQA QPPP AAPA GAPT TTQT NGQG DQQN PAPA GQVD YTKA WEEY YKKM GQAV PAPT GAPP GGQP DYSA AWAE YYRQ QAAY YAQT SPQG MPQH PPAP QGQ
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:100
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:FBP, FUBP, hDH V, FUBP1
Positive Sample:U-87MG, HeLa, HepG2, THP-1, Mouse brain
Cellular Localization:Nucleus.
Calculated MW:68kDa
Observed MW:79kDa

The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID8880
RRIDAB_2766359
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using FUBP1 Rabbit pAb (CAB5587) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human liver damage using FUBP1 Rabbit pAb (CAB5587) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse brain using FUBP1 Rabbit pAb (CAB5587) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of C6 cells using FUBP1 Rabbit pAb (CAB5587) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products