Epigenetics & Nuclear Signaling Antibodies 2

FOXA1 Rabbit Polyclonal Antibody (CAB15278)

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SKU:
CAB15278
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
HNF3A, TCF3A, FOXA1
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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FOXA1 Antibody (CAB15278)

The FOXA1 Antibody (CAB15278) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the FOXA1 protein, this antibody enables precise detection and analysis of FOXA1 expression in various cell types, making it ideal for investigations in molecular biology and cancer research.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:FOXA1 Antibody
SKU:CAB15278
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:QKRF KCEK QPGA GGGG GSGS GGSG AKGG PESR KDPS GASN PSAD SPLH RGVH GKTG QLEG APAP GPAA SPQT LDHS GATA TGGA SELK TPAS STAP PISS GPGA LASV PASH PAHG LAPH ESQL HLKG DPHY SFNH PFSI NNLM SSSE QQHK LDFK AYEQ ALQY SPYG STLP ASLP LGSA SVTT RSPI EPSA LEPA YYQG VYSR PVLN TS
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HNF3A, TCF3A, FOXA1
Positive Sample:Rat testis
Cellular Localization:Nucleus.
Calculated MW:49kDa
Observed MW:50kDa

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

Purification MethodAffinity purification
Gene ID3169
RRIDAB_2762178
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Rat testis, using FOXA1 Rabbit pAb (CAB15278) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat lung using FOXA1 Rabbit pAb (CAB15278) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human breast cancer using FOXA1 Rabbit pAb (CAB15278) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse stomach using FOXA1 Rabbit pAb (CAB15278) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
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