The FMRP Monoclonal Antibody (CAB4539) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using rabbit monoclonal technology, is highly specific and sensitive for detecting FMRP in human samples.FMRP is known to play a crucial role in RNA metabolism and translation regulation, making it essential for proper brain function and neuronal development. Dysregulation of FMRP has been linked to neurodevelopmental disorders such as Fragile X syndrome, autism, and intellectual disabilities.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
FMRP Monoclonal Antibody
SKU:
CAB4539
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1026
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
POF, FMRP, POF1, FRAXA
Positive Sample:
SH-SY5Y, Mouse lung, Mouse brain
Cellular Localization:
Cytoplasm, Nucleus, Nucleolus.
Calculated MW:
71kDa
Observed MW:
70-80kDa
The protein encoded by this gene binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1). Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for this gene.
Purification Method
Affinity purification
Gene ID
2332
RRID
AB_2863292
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using FMRP Rabbit mAb (CAB4539) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Rat testis using FMRP Rabbit mAb (CAB4539) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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