Cardiovascular Antibodies

FGF2 Rabbit Polyclonal Antibody (CAB0235)

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SKU:
CAB0235
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cardiovascular
Reactivity:
Human, Mouse, Rat
Synonyms:
BFGF, FGFB, FGF-2, HBGF-2, FGF2
Research Use:
Cardiovascular
Frequently bought together:

Description

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FGF2 Antibody (CAB0235)

The FGF2 Antibody (CAB0235) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.FGF2, also known as basic fibroblast growth factor, plays a crucial role in tissue repair and regeneration, making it a target of interest in studies related to wound healing, development, and cancer biology.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:FGF2 Antibody
SKU:CAB0235
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:PALP EDGG SGAF PPGH FKDP KRLY CKNG GFFL RIHP DGRV DGVR EKSD PHIK LQLQ AEER GVVS IKGV CANR YLAM KEDG RLLA SKCV TDEC FFFE RLES NNYN TYRS RKYT SWYV ALKR TGQY KLGS KTGP GQKA ILFL PMSA KS
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:BFGF, FGFB, FGF-2, HBGF-2, FGF2
Positive Sample:293T
Cellular Localization:Nucleus, Secreted.
Calculated MW:31kDa
Observed MW:26kDa

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

Purification MethodAffinity purification
Gene ID2247
RRIDAB_2757048
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from 293T cells, using FGF2 Rabbit pAb (CAB0235) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Product ImageWestern blot analysis of various lysates using FGF2 Rabbit pAb (CAB0235) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat ovary using FGF2 Rabbit pAb (CAB0235) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon using FGF2 Rabbit pAb (CAB0235) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of L929 cells using FGF2 Rabbit pAb (CAB0235) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U-2 OS cells using FGF2 Rabbit pAb (CAB0235) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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