Cardiovascular Antibodies

FGF1 Rabbit Polyclonal Antibody (CAB0685)

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SKU:
CAB0685
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cardiovascular
Reactivity:
Human, Mouse, Rat
Synonyms:
AFGF, ECGF, FGFA, ECGFA, ECGFB, FGF-1, HBGF1, HBGF-1, GLIO703, ECGF-beta, FGF-alpha, FGF1
Research Use:
Cardiovascular
Frequently bought together:

Description

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FGF1 Antibody (CAB0685)

The FGF1 Antibody (CAB0685) is a high-quality antibody developed for reliable detection and analysis of target proteins. FGF1 is a key player in cell growth, differentiation, and tissue repair, making it a crucial target for studies in developmental biology, cancer research, and regenerative medicine.This polyclonal antibody, produced in rabbits, is highly specific to human FGF1 and has been validated for use in Western blot applications. It effectively detects FGF1 protein, allowing for detailed analysis in different cell types and tissues. Researchers can rely on this antibody to accurately measure FGF1 levels and investigate its function in various biological contexts.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:FGF1 Antibody
SKU:CAB0685
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:FNLP PGNY KKPK LLYC SNGG HFLR ILPD GTVD GTRD RSDQ HIQL QLSA ESVG EVYI KSTE TGQY LAMD TDGL LYGS QTPN EECL FLER LEEN HYNT YISK KHAE KNWF VGLK KNGS CKRG PRTH YGQK AILF LPLP VSSD
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:100 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:AFGF, ECGF, FGFA, ECGFA, ECGFB, FGF-1, HBGF1, HBGF-1, GLIO703, ECGF-beta, FGF-alpha, FGF1
Positive Sample:U-251MG, Mouse heart, Rat heart
Cellular Localization:Cytoplasm, Nucleus, Secreted, Cell Cortex, Cytosol.
Calculated MW:17kDa
Observed MW:17kDa

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described.

Purification MethodAffinity purification
Gene ID2246
RRIDAB_2757336
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using FGF1 Rabbit pAb (CAB0685) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse brain using FGF1 Rabbit pAb (CAB0685) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of HepG2 cells using FGF1 Rabbit pAb (CAB0685) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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