Epigenetics & Nuclear Signaling Antibodies 1

FEN-1 Rabbit Polyclonal Antibody (CAB1175)

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SKU:
CAB1175
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse
Synonyms:
MF1, RAD2, FEN-1
Research Use:
Epigenetics and Nuclear Signaling
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Description

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FEN-1 Antibody (CAB1175)

The FEN-1 Antibody (CAB1175) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, created by Assay Genie, is specifically designed to target Flap Endonuclease 1 (FEN-1), a key enzyme involved in DNA metabolism.Raised in rabbits, this polyclonal antibody is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the FEN-1 protein, researchers can accurately detect and analyze its expression levels in various cell types. This makes the CAB1175 antibody ideal for studies in molecular biology, genetics, and cancer research.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:FEN-1 Antibody
SKU:CAB1175
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:GDVL QNEE GETT SHLM GMFY RTIR MMEN GIKP VYVF DGKP PQLK SGEL AKRS ERRA EAEK QLQQ AQAA GAEQ EVEK FTKR LVKV TKQH NDEC KHLL SLMG IPYL DAPS EAEA SCAA LVKA GKVY AAAT EDMD CLTF GSPV LMRH LTAS EAKK LPIQ EFHL SRIL QELG LNQE QFVD LCIL LGSD YCES IRGI GPKR AVDL IQKH KSIE EIVR RLDP NKYP VPEN WLHK EAHQ LFLE PEVL DPES VELK WSEP NEEE LIKF MCGE KQFS EERI RSGV KRLS KSRQ GSTQ GRLD DFFK VTGS LSSA KRKE PEPK GSTK KKAK TGAA GKFK RGK
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:10 - 1:100
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:MF1, RAD2, FEN-1
Positive Sample:NIH/3T3, HeLa, Jurkat, Y79, Mouse spleen, Mouse testis
Cellular Localization:Mitochondrion, Nucleus, Nucleolus, Nucleoplasm.
Calculated MW:43kDa
Observed MW:48kDa

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Purification MethodAffinity purification
Gene ID2237
RRIDAB_2758734
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using FEN-1 Rabbit pAb (CAB1175) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human lung cancer using FEN-1 Rabbit pAb (CAB1175) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of A549 cells using FEN-1 Rabbit pAb (CAB1175).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution.
Product ImageImmunofluorescence analysis of HeLa cells using FEN-1 Rabbit pAb (CAB1175).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution.
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