Cell Death Antibodies 2

FADD Rabbit Polyclonal Antibody (CAB5819)

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SKU:
CAB5819
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Reactivity:
Human, Mouse, Rat
Synonyms:
GIG3, IMD90, MORT1, FADD
Research Use:
Cell Death
Frequently bought together:

Description

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FADD Antibody (CAB5819)

The FADD Antibody (CAB5819) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and is suitable for use in Western blot applications. By binding to the FADD protein, this antibody allows for the detection and analysis of FADD in a variety of cell types, making it an essential component of studies in cell biology and cancer research.FADD, or Fas-associated death domain protein, plays a crucial role in regulating programmed cell death and immune responses.

This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:FADD Antibody
SKU:CAB5819
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MDPF LVLL HSVS SSLS SSEL TELK FLCL GRVG KRKL ERVQ SGLD LFSM LLEQ NDLE PGHT ELLR ELLA SLRR HDLL RRVD DFEA GAAA GAAP GEED LCAA FNVI CDNV GKDW RRLA RQLK VSDT KIDS IEDR YPRN LTER VRES LRIW KNTE KENA TVAH LVGA LRSC QMNL VADL VQEV QQAR DLQN RSGA MSPM SWNS DAST SEAS
Tested Applications:WB IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:GIG3, IMD90, MORT1, FADD
Positive Sample:293T, MCF7, HeLa, Mouse liver, Mouse kidney, Mouse heart
Cellular Localization:Cytoplasm, Cytosol, Neuron Projection, Nucleus, Plasma Membrane, Ripoptosome.
Calculated MW:23kDa
Observed MW:26kDa

The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.

Purification MethodAffinity purification
Gene ID8772
RRIDAB_2766571
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using FADD Rabbit pAb (CAB5819) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020).
Product ImageImmunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg FADD antibody (CAB5819). Western blot was performed from the immunoprecipitate using FADD antibody (CAB5819) at a dilution of 1:1000.
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